Multiphoton imaging with high peak power VECSELs

Shamil Mirkhanov; Adrian H. Quarterman; Samuel Swift; Bavishna B. Praveen; Conor J C Smyth; Keith G. Wilcox

doi:10.1117/12.2210862

Multiphoton imaging with high peak power VECSELs

Shamil Mirkhanov, Adrian H. Quarterman, Samuel Swift, Bavishna B. Praveen, Conor J C Smyth, Keith G. Wilcox

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

Original language	English
Title of host publication	Vertical External Cavity Surface Emitting Lasers (VECSELs) VI
Editors	Keith G. Wilcox
Place of Publication	San Francisco
Publisher	SPIE-International Society for Optical Engineering
Volume	9734
ISBN (Electronic)	9781628419696
DOIs	https://doi.org/10.1117/12.2210862
Publication status	Published - 10 Mar 2016
Event	SPIE Photonics West 2016: Vertical External Cavity Surface Emitting Lasers (VECSELs) VI - The Moscone Center, San Francisco, United States Duration: 15 Feb 2016 → 16 Feb 2016 http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016 (Link to Conference details)

Conference

Conference	SPIE Photonics West 2016
Country	United States
City	San Francisco
Period	15/02/16 → 16/02/16
Internet address	http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016 (Link to Conference details)

Keywords

Femtosecond Lasers
Multiphoton Imaging
Multiphoton microscopy
optically pumped semiconducto laser
VECSEL

Access to Document

10.1117/12.2210862

Link to publication in Scopus

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1 Finished

Tuneable Gigahertz Mode Spaced Frequency Combs (Fellowship)
Wilcox, K.
Engineering and Physical Sciences Research Council
1/10/13 → 28/02/18
Project: Research

Cite this

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title = "Multiphoton imaging with high peak power VECSELs",

abstract = "Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.",

keywords = "Femtosecond Lasers, Multiphoton Imaging, Multiphoton microscopy, optically pumped semiconducto laser, VECSEL",

author = "Shamil Mirkhanov and Quarterman, {Adrian H.} and Samuel Swift and Praveen, {Bavishna B.} and Smyth, {Conor J C} and Wilcox, {Keith G.}",

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doi = "10.1117/12.2210862",

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editor = "Wilcox, {Keith G.}",

booktitle = "Vertical External Cavity Surface Emitting Lasers (VECSELs) VI",

publisher = "SPIE-International Society for Optical Engineering",

note = "SPIE Photonics West 2016 : Vertical External Cavity Surface Emitting Lasers (VECSELs) VI ; Conference date: 15-02-2016 Through 16-02-2016",

url = "http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016",

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Mirkhanov, S, Quarterman, AH, Swift, S, Praveen, BB, Smyth, CJC & Wilcox, KG 2016, Multiphoton imaging with high peak power VECSELs. in KG Wilcox (ed.), Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. vol. 9734, 973412, SPIE-International Society for Optical Engineering, San Francisco, SPIE Photonics West 2016, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2210862

Multiphoton imaging with high peak power VECSELs. / Mirkhanov, Shamil; Quarterman, Adrian H.; Swift, Samuel; Praveen, Bavishna B.; Smyth, Conor J C ; Wilcox, Keith G.

Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. ed. / Keith G. Wilcox. Vol. 9734 San Francisco : SPIE-International Society for Optical Engineering, 2016. 973412.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Multiphoton imaging with high peak power VECSELs

AU - Mirkhanov, Shamil

AU - Quarterman, Adrian H.

AU - Swift, Samuel

AU - Praveen, Bavishna B.

AU - Smyth, Conor J C

AU - Wilcox, Keith G.

PY - 2016/3/10

Y1 - 2016/3/10

N2 - Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

AB - Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

KW - Femtosecond Lasers

KW - Multiphoton Imaging

KW - Multiphoton microscopy

KW - optically pumped semiconducto laser

KW - VECSEL

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VL - 9734

BT - Vertical External Cavity Surface Emitting Lasers (VECSELs) VI

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PB - SPIE-International Society for Optical Engineering

CY - San Francisco

T2 - SPIE Photonics West 2016

Y2 - 15 February 2016 through 16 February 2016

ER -

Multiphoton imaging with high peak power VECSELs

Abstract

Conference

Keywords

Access to Document

Tuneable Gigahertz Mode Spaced Frequency Combs (Fellowship)

Cite this