Multiphoton imaging with high peak power VECSELs

Shamil Mirkhanov, Adrian H. Quarterman, Samuel Swift, Bavishna B. Praveen, Conor J C Smyth, Keith G. Wilcox

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

Original languageEnglish
Title of host publicationVertical External Cavity Surface Emitting Lasers (VECSELs) VI
EditorsKeith G. Wilcox
Place of PublicationSan Francisco
PublisherSPIE-International Society for Optical Engineering
Volume9734
ISBN (Electronic)9781628419696
DOIs
Publication statusPublished - 10 Mar 2016
EventSPIE Photonics West 2016: Vertical External Cavity Surface Emitting Lasers (VECSELs) VI - The Moscone Center, San Francisco, United States
Duration: 15 Feb 201616 Feb 2016
http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016 (Link to Conference details)

Conference

ConferenceSPIE Photonics West 2016
CountryUnited States
CitySan Francisco
Period15/02/1616/02/16
Internet address

Fingerprint

Ultrashort pulses
Light sources
Femtosecond Laser
Imaging
Imaging techniques
Microscopy
Wavelength
Fluorophores
Lasers
Aluminum Oxide
Confocal microscopy
Excitation
microscopy
Sapphire
Image quality
Optical microscopy
lasers
Confocal Microscopy
Microscopic examination
light sources

Keywords

  • Femtosecond Lasers
  • Multiphoton Imaging
  • Multiphoton microscopy
  • optically pumped semiconducto laser
  • VECSEL

Cite this

Mirkhanov, S., Quarterman, A. H., Swift, S., Praveen, B. B., Smyth, C. J. C., & Wilcox, K. G. (2016). Multiphoton imaging with high peak power VECSELs. In K. G. Wilcox (Ed.), Vertical External Cavity Surface Emitting Lasers (VECSELs) VI (Vol. 9734). [973412] San Francisco: SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2210862
Mirkhanov, Shamil ; Quarterman, Adrian H. ; Swift, Samuel ; Praveen, Bavishna B. ; Smyth, Conor J C ; Wilcox, Keith G. / Multiphoton imaging with high peak power VECSELs. Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. editor / Keith G. Wilcox. Vol. 9734 San Francisco : SPIE-International Society for Optical Engineering, 2016.
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Mirkhanov, S, Quarterman, AH, Swift, S, Praveen, BB, Smyth, CJC & Wilcox, KG 2016, Multiphoton imaging with high peak power VECSELs. in KG Wilcox (ed.), Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. vol. 9734, 973412, SPIE-International Society for Optical Engineering, San Francisco, SPIE Photonics West 2016, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2210862

Multiphoton imaging with high peak power VECSELs. / Mirkhanov, Shamil; Quarterman, Adrian H.; Swift, Samuel; Praveen, Bavishna B.; Smyth, Conor J C; Wilcox, Keith G.

Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. ed. / Keith G. Wilcox. Vol. 9734 San Francisco : SPIE-International Society for Optical Engineering, 2016. 973412.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

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Mirkhanov S, Quarterman AH, Swift S, Praveen BB, Smyth CJC, Wilcox KG. Multiphoton imaging with high peak power VECSELs. In Wilcox KG, editor, Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. Vol. 9734. San Francisco: SPIE-International Society for Optical Engineering. 2016. 973412 https://doi.org/10.1117/12.2210862