Abstract
Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.
| Original language | English |
|---|---|
| Title of host publication | Vertical External Cavity Surface Emitting Lasers (VECSELs) VI |
| Editors | Keith G. Wilcox |
| Place of Publication | San Francisco |
| Publisher | SPIE-International Society for Optical Engineering |
| Volume | 9734 |
| ISBN (Electronic) | 9781628419696 |
| DOIs | |
| Publication status | Published - 10 Mar 2016 |
| Event | SPIE Photonics West 2016: Vertical External Cavity Surface Emitting Lasers (VECSELs) VI - The Moscone Center, San Francisco, United States Duration: 15 Feb 2016 → 16 Feb 2016 http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016 (Link to Conference details) |
Conference
| Conference | SPIE Photonics West 2016 |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 15/02/16 → 16/02/16 |
| Internet address |
|
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Keywords
- Femtosecond Lasers
- Multiphoton Imaging
- Multiphoton microscopy
- optically pumped semiconducto laser
- VECSEL
Cite this
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Multiphoton imaging with high peak power VECSELs. / Mirkhanov, Shamil; Quarterman, Adrian H.; Swift, Samuel; Praveen, Bavishna B.; Smyth, Conor J C; Wilcox, Keith G.
Vertical External Cavity Surface Emitting Lasers (VECSELs) VI. ed. / Keith G. Wilcox. Vol. 9734 San Francisco : SPIE-International Society for Optical Engineering, 2016. 973412.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Multiphoton imaging with high peak power VECSELs
AU - Mirkhanov, Shamil
AU - Quarterman, Adrian H.
AU - Swift, Samuel
AU - Praveen, Bavishna B.
AU - Smyth, Conor J C
AU - Wilcox, Keith G.
PY - 2016/3/10
Y1 - 2016/3/10
N2 - Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.
AB - Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.
KW - Femtosecond Lasers
KW - Multiphoton Imaging
KW - Multiphoton microscopy
KW - optically pumped semiconducto laser
KW - VECSEL
UR - http://www.scopus.com/inward/record.url?scp=84981217104&partnerID=8YFLogxK
U2 - 10.1117/12.2210862
DO - 10.1117/12.2210862
M3 - Conference contribution
AN - SCOPUS:84981217104
VL - 9734
BT - Vertical External Cavity Surface Emitting Lasers (VECSELs) VI
A2 - Wilcox, Keith G.
PB - SPIE-International Society for Optical Engineering
CY - San Francisco
ER -