Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles

Elizabeth A. Miller, Traude H. Beilharz, Per N. Malkus, Marcus C.S. Lee, Susan Hamamoto, Lelio Orci, Randy Schekman (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

429 Citations (Scopus)

Abstract

We have characterized the mechanisms of cargo selection into ER-derived vesicles by the COPII subunit Sec24p. We identified a site on Sec24p that recognizes the v-SNARE Bet1p and show that packaging of a number of cargo molecules is disrupted when mutations are introduced at this site. Surprisingly, cargo proteins affected by these mutations did not share a single common sorting signal, nor were proteins sharing a putative class of signal affected to the same degree. We show that the same site is conserved as a cargo-interaction domain on the Sec24p homolog Lst1p, which only packages a subset of the cargoes recognized by Sec24p. Finally, we identified an additional mutation that defines another cargo binding domain on Sec24p, which specifically interacts with the SNARE Sec22p. Together, our data support a model whereby Sec24p proteins contain multiple independent cargo binding domains that allow for recognition of a diverse set of sorting signals.

Original languageEnglish
Pages (from-to)497-509
Number of pages13
JournalCell
Volume114
Issue number4
DOIs
Publication statusPublished - 22 Aug 2003

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology

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