Multiple quantum filtered 23Na NMR in the Langendorff perfused mouse heart: Ratio of triple/double quantum filtered signals correlate with [Na]i

Thomas R Eykyn (Lead / Corresponding author), Dunja Aksentijević, Karen L Aughton, Richard Southworth, William Fuller, Michael J Shattock

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    Abstract

    We investigate the potential of multiple quantum filtered (MQF) 23Na NMR to probe intracellular [Na]i in the Langendorff perfused mouse heart. In the presence of Tm(DOTP) shift reagent the triple quantum filtered (TQF) signal originated largely from the intracellular sodium pool with a 32±6 % contribution of the total TQF signal arising from extracellular sodium, whilst the rank 2 double-quantum filtered signal (DQF), acquired with a 54.7° flip-angle pulse, originated exclusively from the extracellular sodium pool. Given the different cellular origins of the 23Na MQF signals we propose that the TQF/DQF ratio can be used as a semi-quantitative measure of [Na]i in the mouse heart. We demonstrate a good correlation of this ratio with [Na]i measured with shift reagent at baseline and under conditions of elevated [Na]i. We compare the measurements of [Na]i using both shift reagent and TQF/DQF ratio in a cohort of wild type mouse hearts and in a transgenic PLM3SA mouse expressing a non-phosphorylatable form of phospholemman, showing a modest but measurable elevation of baseline [Na]i. MQF filtered 23Na NMR is a potentially useful tool for studying normal and pathophysiological changes in [Na]i, particularly in transgenic mouse models with altered Na regulation.

    Original languageEnglish
    Pages (from-to)95-101
    Number of pages7
    JournalJournal of Molecular and Cellular Cardiology
    Volume86
    Early online date18 Jul 2015
    DOIs
    Publication statusPublished - 1 Sep 2015

    Keywords

    • Multiple quantum filtered 23Na
    • TQF
    • DQF
    • Langendorff perfused mouse heart
    • Shift reagent

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