TY - JOUR
T1 - Mutations of APC, K-ras, and p53 are associated with specific chromosomal aberrations in colorectal adenocarcinomas
AU - Leslie, Amy
AU - Pratt, Norman R.
AU - Gillespie, Karen
AU - Sales, Mark
AU - Kernohan, Neil M.
AU - Smith, Gillian
AU - Wolf, C. Roland
AU - Carey, Francis A.
AU - Steele, Robert J. C.
N1 - dc.publisher: AACR Publications
Senior author of paper describing study of 50 colorectal adenocarcinomas in which mutation of p53 is associated with gain of 20q, 13q, and 8q and loss of 18q. APC mutation was associated with gain of 7p and gain of 12p, although less common, was significantly associated with K-ras mutation.
PY - 2003/8
Y1 - 2003/8
N2 - It is widely accepted that both large-scale chromosomal abnormalities and mutation of specific genes, such as APC, K-ras, and/or p53, occur in the majority of colorectal adenocarcinomas. Whether or not a relationship exists between these different forms of genetic abnormalities was previously unknown. Using comparative genomic hybridization and mutational analysis of APC, K-ras, and p53 to evaluate 50 colorectal adenocarcinomas, we have shown that mutation of p53 is significantly associated with gain of 20q, 13q, and 8q and loss of 18q (P = 0.000, 0.02, 0.044, and 0.001, respectively). Conversely, APC mutation did not associate with any of the above-mentioned aberrations but did associate significantly with gain of 7p (P = 0.01). Gain of chromosomal arm 12p, although a less common aberration, was significantly associated with K-ras mutation (P = 0.011). The associations we have described should refine the search for candidate genes underlying chromosomal aberrations and assist in the definition of distinct pathways in colorectal tumorigenesis.
AB - It is widely accepted that both large-scale chromosomal abnormalities and mutation of specific genes, such as APC, K-ras, and/or p53, occur in the majority of colorectal adenocarcinomas. Whether or not a relationship exists between these different forms of genetic abnormalities was previously unknown. Using comparative genomic hybridization and mutational analysis of APC, K-ras, and p53 to evaluate 50 colorectal adenocarcinomas, we have shown that mutation of p53 is significantly associated with gain of 20q, 13q, and 8q and loss of 18q (P = 0.000, 0.02, 0.044, and 0.001, respectively). Conversely, APC mutation did not associate with any of the above-mentioned aberrations but did associate significantly with gain of 7p (P = 0.01). Gain of chromosomal arm 12p, although a less common aberration, was significantly associated with K-ras mutation (P = 0.011). The associations we have described should refine the search for candidate genes underlying chromosomal aberrations and assist in the definition of distinct pathways in colorectal tumorigenesis.
M3 - Article
SN - 0008-5472
VL - 63
SP - 4656
EP - 4661
JO - Cancer Research
JF - Cancer Research
IS - 15
ER -