mVps34 is activated following high-resistance contractions

Matthew G. MacKenzie, D. Lee Hamilton, James T. Murray, Peter M. Taylor, Keith Baar

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 +/- 112.08%, 3 h = 124.7 +/- 15.96% and 6 h = 129.2 +/- 0%) and mVps34 (3 h = 68.8 +/- 15.1% and 6 h = 36.0 +/- 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 +/- 2.29% and 3 h = 47.0 +/- 6.65%; mVps34 3 h = 24.5 +/- 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.

    Original languageEnglish
    Pages (from-to)253-260
    Number of pages8
    JournalJournal of Physiology
    Volume587
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2009

    Keywords

    • SKELETAL-MUSCLE HYPERTROPHY
    • RAPAMYCIN-DEPENDENT MANNER
    • EXERCISE-INDUCED INCREASE
    • AMINO-ACID-TRANSPORT
    • PROTEIN-SYNTHESIS
    • SACCHAROMYCES-CEREVISIAE
    • MAMMALIAN TARGET
    • KINASE
    • GROWTH
    • 3-KINASE

    Cite this

    MacKenzie, M. G., Hamilton, D. L., Murray, J. T., Taylor, P. M., & Baar, K. (2009). mVps34 is activated following high-resistance contractions. Journal of Physiology, 587(1), 253-260. https://doi.org/10.1113/jphysiol.2008.159830
    MacKenzie, Matthew G. ; Hamilton, D. Lee ; Murray, James T. ; Taylor, Peter M. ; Baar, Keith. / mVps34 is activated following high-resistance contractions. In: Journal of Physiology. 2009 ; Vol. 587, No. 1. pp. 253-260.
    @article{8a34fc8b9bc74ba187f28a1afeb77cd4,
    title = "mVps34 is activated following high-resistance contractions",
    abstract = "Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 +/- 112.08{\%}, 3 h = 124.7 +/- 15.96{\%} and 6 h = 129.2 +/- 0{\%}) and mVps34 (3 h = 68.8 +/- 15.1{\%} and 6 h = 36.0 +/- 8.79{\%}) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 +/- 2.29{\%} and 3 h = 47.0 +/- 6.65{\%}; mVps34 3 h = 24.5 +/- 7.92{\%}). HPLC analysis of the TA demonstrated a 25{\%} increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.",
    keywords = "SKELETAL-MUSCLE HYPERTROPHY, RAPAMYCIN-DEPENDENT MANNER, EXERCISE-INDUCED INCREASE, AMINO-ACID-TRANSPORT, PROTEIN-SYNTHESIS, SACCHAROMYCES-CEREVISIAE, MAMMALIAN TARGET, KINASE, GROWTH, 3-KINASE",
    author = "MacKenzie, {Matthew G.} and Hamilton, {D. Lee} and Murray, {James T.} and Taylor, {Peter M.} and Keith Baar",
    year = "2009",
    month = "1",
    day = "1",
    doi = "10.1113/jphysiol.2008.159830",
    language = "English",
    volume = "587",
    pages = "253--260",
    journal = "Journal of Physiology",
    issn = "0022-3751",
    publisher = "Wiley",
    number = "1",

    }

    MacKenzie, MG, Hamilton, DL, Murray, JT, Taylor, PM & Baar, K 2009, 'mVps34 is activated following high-resistance contractions', Journal of Physiology, vol. 587, no. 1, pp. 253-260. https://doi.org/10.1113/jphysiol.2008.159830

    mVps34 is activated following high-resistance contractions. / MacKenzie, Matthew G.; Hamilton, D. Lee; Murray, James T.; Taylor, Peter M.; Baar, Keith.

    In: Journal of Physiology, Vol. 587, No. 1, 01.01.2009, p. 253-260.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - mVps34 is activated following high-resistance contractions

    AU - MacKenzie, Matthew G.

    AU - Hamilton, D. Lee

    AU - Murray, James T.

    AU - Taylor, Peter M.

    AU - Baar, Keith

    PY - 2009/1/1

    Y1 - 2009/1/1

    N2 - Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 +/- 112.08%, 3 h = 124.7 +/- 15.96% and 6 h = 129.2 +/- 0%) and mVps34 (3 h = 68.8 +/- 15.1% and 6 h = 36.0 +/- 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 +/- 2.29% and 3 h = 47.0 +/- 6.65%; mVps34 3 h = 24.5 +/- 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.

    AB - Following resistance exercise in the fasted state, both protein synthesis and degradation in skeletal muscle are increased. The addition of essential amino acids potentiates the synthetic response suggesting that an amino acid sensor, which is involved in both synthesis and degradation, may be activated by resistance exercise. One such candidate protein is the class 3 phosphatidylinositol 3OH-kinase (PI3K) Vps34. To determine whether mammalian Vps34 (mVps34) is modulated by high-resistance contractions, mVps34 and S6K1 (an index of mTORC1) activity were measured in the distal hindlimb muscles of rats 0.5, 3, 6 and 18 h after acute unilateral high-resistance contractions with the contralateral muscles serving as a control. In the lengthening tibialis anterior (TA) muscle, S6K1 (0.5 h = 366.3 +/- 112.08%, 3 h = 124.7 +/- 15.96% and 6 h = 129.2 +/- 0%) and mVps34 (3 h = 68.8 +/- 15.1% and 6 h = 36.0 +/- 8.79%) activity both increased, whereas in the shortening soleus and plantaris (PLN) muscles the increase was significantly lower (PLN S6K1 0.5 h = 33.1 +/- 2.29% and 3 h = 47.0 +/- 6.65%; mVps34 3 h = 24.5 +/- 7.92%). HPLC analysis of the TA demonstrated a 25% increase in intramuscular leucine concentration in rats 1.5 h after exercise. A similar level of leucine added to C2C12 cells in vitro increased mVps34 activity 3.2-fold. These data suggest that, following high-resistance contractions, mVps34 activity is stimulated by an influx of essential amino acids such as leucine and this may prolong mTORC1 signalling and contribute to muscle hypertrophy.

    KW - SKELETAL-MUSCLE HYPERTROPHY

    KW - RAPAMYCIN-DEPENDENT MANNER

    KW - EXERCISE-INDUCED INCREASE

    KW - AMINO-ACID-TRANSPORT

    KW - PROTEIN-SYNTHESIS

    KW - SACCHAROMYCES-CEREVISIAE

    KW - MAMMALIAN TARGET

    KW - KINASE

    KW - GROWTH

    KW - 3-KINASE

    U2 - 10.1113/jphysiol.2008.159830

    DO - 10.1113/jphysiol.2008.159830

    M3 - Article

    VL - 587

    SP - 253

    EP - 260

    JO - Journal of Physiology

    JF - Journal of Physiology

    SN - 0022-3751

    IS - 1

    ER -

    MacKenzie MG, Hamilton DL, Murray JT, Taylor PM, Baar K. mVps34 is activated following high-resistance contractions. Journal of Physiology. 2009 Jan 1;587(1):253-260. https://doi.org/10.1113/jphysiol.2008.159830