Abstract
The c-myc proto-oncogene regulates the expression of 15% to 20% of all genes, depending on the cell type, and the regulation is usually odest (1.5- to 2.0-fold). The authors discovered that in addition to regulating mRNA abundance, c-Myc regulates the formation of the 7-methylguanosine cap on many mRNAs, including transcriptional target genes and others not transcriptionally activated. Because the 7-methylguanosine cap is required for effective translation, enhanced methyl cap formation leads to increased protein production from Myc-responsive genes that exceeds the transcriptional induction. Increased cap methylation is linked to Myc-dependent enhanced activity of 2 critical kinases, TFIIH and p-TEFb, which phosphorylate the RNA polymerase II carboxy-terminal domain (CTD). Phosphorylation of the CTD recruits RNGTT and RNMT, the enzymes involved in mRNA capping, to the nascent transcript. Evidence is accumulating that enhanced cap methylation makes a significant contribution to Mycdependent gene regulation and protein production.
Original language | English |
---|---|
Pages (from-to) | 576-579 |
Number of pages | 4 |
Journal | Genes and Cancer |
Volume | 1 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2010 |
Keywords
- Myc
- Gene expression
- Translation
- Cap methylation
- Cell proliferation