N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

Ziqiang Guan, Antonia Delago, Phillip Nußbaum, Benjamin Meyer, Sonja Verena Albers, Jerry Eichler (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
176 Downloads (Pure)


N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

Original languageEnglish
Pages (from-to)3168-3178
Number of pages11
JournalFEBS Letters
Issue number18
Early online date4 Aug 2016
Publication statusPublished - 12 Sep 2016


  • Sulfolobus acidocaldarius
  • Archaea
  • Dolichol
  • N-glycosylation
  • Thermoacidophile

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