N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

TY - JOUR

T1 - N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

AU - Guan, Ziqiang

AU - Delago, Antonia

AU - Nußbaum, Phillip

AU - Meyer, Benjamin

AU - Albers, Sonja Verena

AU - Eichler, Jerry

N1 - Funded by LIPID MAPS Large Scale Collaborative. Grant Number: GM-069338 NIH. Grant Number: EY023666. German-Israeli Foundation for Scientific Research and Development. Grant Number: I-1290-416.13/2015. ERC. Grant Number: 311523

PY - 2016/9/12

Y1 - 2016/9/12

N2 - N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

AB - N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

KW - Sulfolobus acidocaldarius

KW - Archaea

KW - Dolichol

KW - N-glycosylation

KW - Thermoacidophile

UR - https://www.scopus.com/pages/publications/84987834902

U2 - 10.1002/1873-3468.12341

DO - 10.1002/1873-3468.12341

M3 - Article

C2 - 27490243

AN - SCOPUS:84987834902

SN - 0014-5793

VL - 590

SP - 3168

EP - 3178

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -