TY - JOUR
T1 - Nano-topography
T2 - Quicksand for cell cycle progression?
AU - Giannini, Marianna
AU - Primerano, Chiara
AU - Berger, Liron
AU - Giannaccini, Martina
AU - Wang, Zhigang
AU - Landi, Elena
AU - Cuschieri, Alfred
AU - Dente, Luciana
AU - Signore, Giovanni
AU - Raffa, Vittoria
N1 - Copyright © 2018 Elsevier Inc. All rights reserved.
PY - 2018/11
Y1 - 2018/11
N2 - The 3-D spatial and mechanical features of nano-topography can create alternative environments, which influence cellular response. In this paper, murine fibroblast cells were grown on surfaces characterized by protruding nanotubes. Cells cultured on such nano-structured surface exhibit stronger cellular adhesion compared to control groups, but despite the fact that stronger adhesion is generally believed to promote cell cycle progression, the time cells spend in G1 phase is doubled. This apparent contradiction is solved by confocal microscopy analysis, which shows that the nano-topography inhibits actin stress fiber formation. In turn, this impairs RhoA activation, which is required to suppress the inhibition of cell cycle progression imposed by p21/p27. This finding suggests that the generation of stress fibers, required to impose the homeostatic intracellular tension, rather than cell adhesion/spreading is the limiting factor for cell cycle progression. Indeed, nano-topography could represent a unique tool to inhibit proliferation in adherent well-spread cells.
AB - The 3-D spatial and mechanical features of nano-topography can create alternative environments, which influence cellular response. In this paper, murine fibroblast cells were grown on surfaces characterized by protruding nanotubes. Cells cultured on such nano-structured surface exhibit stronger cellular adhesion compared to control groups, but despite the fact that stronger adhesion is generally believed to promote cell cycle progression, the time cells spend in G1 phase is doubled. This apparent contradiction is solved by confocal microscopy analysis, which shows that the nano-topography inhibits actin stress fiber formation. In turn, this impairs RhoA activation, which is required to suppress the inhibition of cell cycle progression imposed by p21/p27. This finding suggests that the generation of stress fibers, required to impose the homeostatic intracellular tension, rather than cell adhesion/spreading is the limiting factor for cell cycle progression. Indeed, nano-topography could represent a unique tool to inhibit proliferation in adherent well-spread cells.
KW - Cell cyle
KW - Mechanotransduction
KW - Nano-topography
KW - RhoA
U2 - 10.1016/j.nano.2018.07.002
DO - 10.1016/j.nano.2018.07.002
M3 - Article
C2 - 30010000
AN - SCOPUS:85050386504
VL - 14
SP - 2656
EP - 2665
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
SN - 1549-9634
IS - 8
ER -