Native detection of protein O-GlcNAcylation by gel electrophoresis

Chuan Fu, Daan M. F. van Aalten (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)
    36 Downloads (Pure)


    O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.
    Original languageEnglish
    Pages (from-to)6826-6830
    Number of pages5
    Issue number21
    Early online date2 Sept 2020
    Publication statusPublished - 7 Nov 2020

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry


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