Native detection of protein O-GlcNAcylation by gel electrophoresis

Chuan Fu, Daan M. F. van Aalten (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.
Original languageEnglish
Pages (from-to)6826-6830
Number of pages5
JournalAnalyst
Volume145
Issue number21
Early online date2 Sep 2020
DOIs
Publication statusPublished - 7 Nov 2020

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