Native detection of protein O-GlcNAcylation by gel electrophoresis

Chuan Fu; Daan M. F.  van Aalten

doi:10.1039/C9AN02506E

Native detection of protein O-GlcNAcylation by gel electrophoresis

Chuan Fu, Daan M. F. van Aalten (Lead / Corresponding author)

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Abstract

O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

Original language	English
Pages (from-to)	6826-6830
Number of pages	5
Journal	Analyst
Volume	145
Issue number	21
Early online date	2 Sept 2020
DOIs	https://doi.org/10.1039/C9AN02506E
Publication status	Published - 7 Nov 2020

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/C9AN02506ELicence: CC BY

Final Published VersionFinal published version, 1.02 MBLicence: CC BY

Cite this

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title = "Native detection of protein O-GlcNAcylation by gel electrophoresis",

abstract = "O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.",

author = "Chuan Fu and {van Aalten}, {Daan M. F.}",

note = "This work was funded by a Wellcome Trust Senior Research Fellowship (110061)",

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AU - Fu, Chuan

AU - van Aalten, Daan M. F.

N1 - This work was funded by a Wellcome Trust Senior Research Fellowship (110061)

PY - 2020/11/7

Y1 - 2020/11/7

N2 - O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

AB - O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of OGlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

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U2 - 10.1039/C9AN02506E

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Native detection of protein O-GlcNAcylation by gel electrophoresis

Abstract

ASJC Scopus subject areas

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Molecular Mechanisms of O-GICNAC Signalling (Investigator award)

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Native detection of protein O-GlcNAcylation by gel electrophoresis

Abstract

ASJC Scopus subject areas

Access to Document

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Molecular Mechanisms of O-GICNAC Signalling (Investigator award)

Cite this