Natural abundance 13C-nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress

Amanda J. Meikle; John A. Chudek; Robert H. Reed; Geoffrey M. Gadd

doi:10.1111/j.1574-6968.1991.tb04859.x

Natural abundance ¹³C-nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress

Amanda J. Meikle
, John A. Chudek
, Robert H. Reed
, Geoffrey M. Gadd (Lead / Corresponding author)

University of Dundee

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Analysis of seventeen yeast strains by ¹³C-NMR spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt-stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. Ribitol was detected in some species, including Debaryomyces hansenii, although ribitol accumulation did not correlate with the osmotic pressure of the medium. Relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmotic pressure was increased. Trehalose was present during exponential growth of some species.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	82
Issue number	2
Early online date	12 May 1991
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04859.x
Publication status	Published - 1 Aug 1991

Keywords

C-Nuclear magnetic resonance
Arabitol
Glycerol
Osmotolerance
Spectroscopy
Trehalose
Yeast

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1991.tb04859.x

Cite this

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title = "Natural abundance 13C-nuclear magnetic resonance spectroscopic analysis of acyclic polyol and trehalose accumulation by several yeast species in response to salt stress",

abstract = "Analysis of seventeen yeast strains by 13C-NMR spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt-stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. Ribitol was detected in some species, including Debaryomyces hansenii, although ribitol accumulation did not correlate with the osmotic pressure of the medium. Relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmotic pressure was increased. Trehalose was present during exponential growth of some species.",

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AU - Reed, Robert H.

AU - Gadd, Geoffrey M.

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AB - Analysis of seventeen yeast strains by 13C-NMR spectroscopy has confirmed the significance of glycerol as the sole osmoregulatory solute under salt-stressed conditions, and has shown arabitol to be present in most of the osmotolerant species. Ribitol was detected in some species, including Debaryomyces hansenii, although ribitol accumulation did not correlate with the osmotic pressure of the medium. Relative amounts of arabitol and ribitol decreased in relation to glycerol when the external osmotic pressure was increased. Trehalose was present during exponential growth of some species.

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KW - Arabitol

KW - Glycerol

KW - Osmotolerance

KW - Spectroscopy

KW - Trehalose

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