Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Ryan J. Morris; Marieke Schor; Rachel M. C. Gillespie; Ana Sofia Ferreira; Lucia Baldauf; Chris Earl; Adam Ostrowski; Laura Hobley; Keith M. Bromley; Tetyana Sukhodub; Sofia Arnaouteli; Nicola R. Stanley-Wall; Cait E. MacPhee

doi:10.1038/s41598-017-06786-9

Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Ryan J. Morris, Marieke Schor, Rachel M. C. Gillespie, Ana Sofia Ferreira, Lucia Baldauf, Chris Earl, Adam Ostrowski, Laura Hobley, Keith M. Bromley, Tetyana Sukhodub, Sofia Arnaouteli, Nicola R. Stanley-Wall, Cait E. MacPhee (Lead / Corresponding author)

Molecular Microbiology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

237 Downloads (Pure)

Abstract

BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

Original language	English
Article number	6730
Pages (from-to)	1-13
Number of pages	13
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/s41598-017-06786-9
Publication status	Published - 27 Jul 2017

Keywords

Biofilms
Biological physics
Supramolecular assembly

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10.1038/s41598-017-06786-9Licence: CC BY

Final Published Version
© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 5.91 MBLicence: CC BY

Architecture of a Biofilm (Joint with University of Edinburgh)
Campbell, P. (Investigator), Davidson, F. (Investigator), Ferguson, M. (Investigator), Stanley-Wall, N. (Investigator) & Swedlow, J. (Investigator)
Biotechnology and Biological Sciences Research Council
1/02/17 → 31/07/23
Project: Research
Biology and Physics at the Biofilm Surface (Joint with University of Edinburgh)
Stanley-Wall, N. (Investigator) & van Aalten, D. (Investigator)
Biotechnology and Biological Sciences Research Council
31/03/14 → 30/03/17
Project: Research
How to Build a Biofilm
Stanley-Wall, N. (Investigator) & van Aalten, D. (Investigator)
1/01/12 → 30/04/15
Project: Research

Understanding the role of TapA in the formation of B. subtilis biofilms: a biochemical and genetic analysis
Earl, C. (Author), Stanley-Wall, N. (Supervisor) & Coulthurst, S. (Supervisor), 2018
Student thesis: Doctoral Thesis › Doctor of Philosophy

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Morris, R. J., Schor, M., Gillespie, R. M. C., Ferreira, A. S., Baldauf, L., Earl, C., Ostrowski, A., Hobley, L., Bromley, K. M., Sukhodub, T., Arnaouteli, S., Stanley-Wall, N. R., & MacPhee, C. E. (2017). Natural variations in the biofilm-associated protein BslA from the genus Bacillus. Scientific Reports, 7, 1-13. Article 6730. https://doi.org/10.1038/s41598-017-06786-9

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title = "Natural variations in the biofilm-associated protein BslA from the genus Bacillus",

abstract = "BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.",

keywords = "Biofilms, Biological physics, Supramolecular assembly",

author = "Morris, {Ryan J.} and Marieke Schor and Gillespie, {Rachel M. C.} and Ferreira, {Ana Sofia} and Lucia Baldauf and Chris Earl and Adam Ostrowski and Laura Hobley and Bromley, {Keith M.} and Tetyana Sukhodub and Sofia Arnaouteli and Stanley-Wall, {Nicola R.} and MacPhee, {Cait E.}",

note = "This work has been supported by funding from the Engineering and Physical Sciences Research Council [EP/J007404/1] and the Biotechnology and Biological Sciences Research Council [BB/L006979/1; BB/I019464/1; BB/L006804/1].",

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journal = "Scientific Reports",

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TY - JOUR

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AU - Morris, Ryan J.

AU - Schor, Marieke

AU - Gillespie, Rachel M. C.

AU - Ferreira, Ana Sofia

AU - Baldauf, Lucia

AU - Earl, Chris

AU - Ostrowski, Adam

AU - Hobley, Laura

AU - Bromley, Keith M.

AU - Sukhodub, Tetyana

AU - Arnaouteli, Sofia

AU - Stanley-Wall, Nicola R.

AU - MacPhee, Cait E.

N1 - This work has been supported by funding from the Engineering and Physical Sciences Research Council [EP/J007404/1] and the Biotechnology and Biological Sciences Research Council [BB/L006979/1; BB/I019464/1; BB/L006804/1].

PY - 2017/7/27

Y1 - 2017/7/27

N2 - BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

AB - BslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

KW - Biofilms

KW - Biological physics

KW - Supramolecular assembly

U2 - 10.1038/s41598-017-06786-9

DO - 10.1038/s41598-017-06786-9

M3 - Article

C2 - 28751732

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SP - 1

EP - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 6730

ER -

Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Abstract

Keywords

Access to Document

Fingerprint

Architecture of a Biofilm (Joint with University of Edinburgh)

Biology and Physics at the Biofilm Surface (Joint with University of Edinburgh)

How to Build a Biofilm

Understanding the role of TapA in the formation of B. subtilis biofilms: a biochemical and genetic analysis

Cite this

Natural variations in the biofilm-associated protein BslA from the genus Bacillus

Abstract

Keywords

Access to Document

Fingerprint

Projects

Architecture of a Biofilm (Joint with University of Edinburgh)

Biology and Physics at the Biofilm Surface (Joint with University of Edinburgh)

How to Build a Biofilm

Student theses

Understanding the role of TapA in the formation of B. subtilis biofilms: a biochemical and genetic analysis

Cite this