NEDD8 overexpression results in neddylation of ubiquitin substrates by the ubiquitin pathway

Roland Hjerpe, Yann Thomas, Thimo Kurz

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    Ubiquitin and ubiquitin-like proteins use unique E1, E2, and E3 enzymes for conjugation to their substrates. We and others have recently reported that increases in the relative concentration of the ubiquitin-like protein NEDD8 over ubiquitin lead to activation of NEDD8 by the ubiquitin E1 enzyme. We now show that this results in erroneous conjugation of NEDD8 to ubiquitin substrates, such as p53, Caspase 7, and Hif1a, demonstrating that overexpression of NEDD8 is not appropriate for identification of substrates of the NEDD8 pathway.
    Original languageEnglish
    Pages (from-to)27-9
    Number of pages3
    JournalJournal of Molecular Biology
    Volume421
    Issue number1
    DOIs
    Publication statusPublished - Aug 2012

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