TY - JOUR
T1 - NEDD8 overexpression results in neddylation of ubiquitin substrates by the ubiquitin pathway
AU - Hjerpe, Roland
AU - Thomas, Yann
AU - Kurz, Thimo
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2012/8
Y1 - 2012/8
N2 - Ubiquitin and ubiquitin-like proteins use unique E1, E2, and E3 enzymes for conjugation to their substrates. We and others have recently reported that increases in the relative concentration of the ubiquitin-like protein NEDD8 over ubiquitin lead to activation of NEDD8 by the ubiquitin E1 enzyme. We now show that this results in erroneous conjugation of NEDD8 to ubiquitin substrates, such as p53, Caspase 7, and Hif1a, demonstrating that overexpression of NEDD8 is not appropriate for identification of substrates of the NEDD8 pathway.
AB - Ubiquitin and ubiquitin-like proteins use unique E1, E2, and E3 enzymes for conjugation to their substrates. We and others have recently reported that increases in the relative concentration of the ubiquitin-like protein NEDD8 over ubiquitin lead to activation of NEDD8 by the ubiquitin E1 enzyme. We now show that this results in erroneous conjugation of NEDD8 to ubiquitin substrates, such as p53, Caspase 7, and Hif1a, demonstrating that overexpression of NEDD8 is not appropriate for identification of substrates of the NEDD8 pathway.
U2 - 10.1016/j.jmb.2012.05.013
DO - 10.1016/j.jmb.2012.05.013
M3 - Article
C2 - 22608973
VL - 421
SP - 27
EP - 29
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -