Need for speed: Examining protein behaviour during cryoEM grid preparation at different timescales

David P. Klebl, Molly S. C. Gravett, Dimitrios Kontziampasis, David J. Wright, Robin S. Bon, Diana C. F. Monteiro, Martin Trebbin, Frank Sobott, Howard D. White, Michele C. Darrow, Rebecca F. Thompson (Lead / Corresponding author), Stephen P. Muench (Lead / Corresponding author)

Research output: Working paper/PreprintPreprint

Abstract

A host of new technologies are under development to improve the quality and reproducibility of cryoEM grid preparation. Here we have systematically investigated the preparation of three macromolecular complexes using three different vitrification devices (Vitrobot™, chameleon and a time-resolved cryoEM device) on various timescales, including grids made within 6 ms, (the fastest reported to date), to interrogate particle behaviour at the air-water interface for different timepoints. Results demonstrate that different macromolecular complexes can respond to the thin film environment formed during cryoEM sample preparation in highly variable ways, shedding light on why cryoEM sample preparation can be difficult to optimise. We demonstrate that reducing time between sample application and vitrification is just one tool to improve cryoEM grid quality, but that it is unlikely to be a generic ‘silver bullet’ for improving the quality of every cryoEM sample preparation.

Original languageEnglish
PublisherBioRxiv
Pages1-33
Number of pages33
DOIs
Publication statusPublished - 15 May 2020

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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