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Abstract
Robust protocols for directed differentiation of human pluripotent cells are required to determine whether mechanisms operating in model organisms are relevant to our own development. Recent work in vertebrate embryos has identified neuromesodermal progenitors as a bipotent cell population that contributes to paraxial mesoderm and spinal cord. However, precise protocols for in vitro differentiation of human spinal cord progenitors are lacking. Informed by signalling in amniote embryos, we show here that transient dual-SMAD inhibition, together with retinoic acid (dSMADi-RA), provides rapid and reproducible induction of human spinal cord progenitors from neuromesodermal progenitor-like cells. Using CRISPR-Cas9 to engineer human embryonic stem cells with a GFP-reporter for neuromesodermal progenitor-associated gene Nkx1.2 we facilitate selection of this cell population. RNA-sequencing was then used to identify human and conserved neuromesodermal progenitor transcriptional signatures, to validate this differentiation protocol and to reveal new pathways/processes in human neural differentiation. This optimised protocol, novel reporter line and transcriptomic data are useful resources with which to dissect molecular mechanisms regulating human spinal cord generation and allow the scaling-up of distinct cell populations for global analyses, including proteomic, biochemical and chromatin interrogation.
Original language | English |
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Article number | dev166215 |
Number of pages | 10 |
Journal | Development |
Volume | 145 |
Issue number | 16 |
Early online date | 13 Jun 2018 |
DOIs | |
Publication status | Published - 12 Jul 2018 |
Keywords
- neuromesodermal progenitor-like cells
- human neural development
- human spinal cord
- dual SMAD inhibition
- CRISPR-Cas9
- human ES cells
- Nkx1.2 reporter
- human neuromesodermal progenitor transcriptome
- Human neuromesodermal progenitor transcriptome
- Human neural development
- Neuromesodermal progenitor-like cells
- Human ES cells
- Human spinal cord
- Dual SMAD inhibition
- CRISPR-cas9
- Embryo, Mammalian
- Humans
- Gene Expression Profiling
- Body Patterning/genetics
- Gene Expression Regulation, Developmental
- Neural Stem Cells/cytology
- Stem Cells/cytology
- Cell Lineage/genetics
- Cells, Cultured
- Spinal Cord/cytology
- Animals
- Cell Differentiation/genetics
- Mice
- Mesoderm/cytology
- Neurogenesis/genetics
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
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- 1 Finished
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Cellular and Molecular Mechanisms Regulating Neuronal Differentiation in Embryos and Adults (Senior Investigator award)
Storey, K. (Investigator)
21/07/14 → 31/12/21
Project: Research