Neutron reflectivity as a tool for physics-based studies of model bacterial membranes

Robert D. Barker, Laura E. McKinley, Simon Titmuss (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

6 Citations (Scopus)


The principles of neutron reflectivity and its application as a tool to provide structural information at the (sub-) molecular unit length scale from models for bacterial membranes are described. The model membranes can take the form of a monolayer for a single leaflet spread at the air/water interface, or bilayers of increasing complexity at the solid/liquid interface. Solid-supported bilayers constrain the bilayer to 2D but can be used to characterize interactions with antimicrobial peptides and benchmark high throughput lab-based techniques. Floating bilayers allow for membrane fluctuations, making the phase behaviour more representative of native membranes. Bilayers of varying levels of compositional accuracy can now be constructed, facilitating studies with aims that range from characterizing the fundamental physical interactions, through to the characterization of accurate mimetics for the inner and outer membranes of Gram-negative bacteria. Studies of the interactions of antimicrobial peptides with monolayer and bilayer models for the inner and outer membranes have revealed information about the molecular control of the outer membrane permeability, and the mode of interaction of antimicrobials with both inner and outer membranes.

Original languageEnglish
Title of host publicationBiophysics of infection
EditorsMark C. Leake
PublisherSpringer International Publishing
Number of pages22
ISBN (Electronic)9783319321899
ISBN (Print)9783319321875
Publication statusPublished - 19 May 2016

Publication series

NameAdvances in experimental medicine and biology
PublisherSpringer International Publishing
ISSN (Print)0065-2598


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