Non-Invasive Intravital Imaging of siRNA-Mediated Mutant Keratin Gene Repression in Skin

Robyn P. Hickerson, Tycho J. Speaker, Maria Fernanda Lara, Emilio González-González, Manuel A. Flores, Christopher H. Contag, Roger L. Kaspar (Lead / Corresponding author)

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Small interfering RNAs (siRNAs) specifically and potently inhibit target gene expression. Pachyonychia congenita (PC) is a skin disorder caused by mutations in genes encoding keratin (K) 6a/b, K16, and K17, resulting in faulty intermediate filaments. A siRNA targeting a single nucleotide, PC-relevant mutation inhibits K6a expression and has been evaluated in the clinic with encouraging results. Procedures: To better understand the pathophysiology of PC, and develop a model system to study siRNA delivery and visualize efficacy in skin, wild type (WT) and mutant K6a complementary DNAs (cDNAs) were fused to either enhanced green fluorescent protein or tandem tomato fluorescent protein cDNA to allow covisualization of mutant and WT K6a expression in mouse footpad skin using a dual fluorescence in vivo confocal imaging system equipped with 488 and 532 nm lasers. Results: Expression of mutant K6a/reporter resulted in visualization of keratin aggregates, while expression of WT K6a/reporter led to incorporation into filaments. Addition of mutant K6a-specific siRNA resulted in inhibition of mutant, but not WT, K6a/reporter expression. Conclusions: Intravital imaging offers subcellular resolution for tracking functional activity of siRNA in real time and enables detailed analyses of therapeutic effects in individual mice to facilitate development of nucleic acid-based therapeutics for skin disorders.

Original languageEnglish
Pages (from-to)34-42
Number of pages9
JournalMolecular Imaging and Biology
Volume18
Issue number1
Early online date14 Jul 2015
DOIs
Publication statusPublished - Feb 2016

    Fingerprint

Keywords

  • Gene regulation
  • Gene therapy
  • Genodermatosis
  • In vivo confocal fluorescence microscopy

Cite this

Hickerson, R. P., Speaker, T. J., Lara, M. F., González-González, E., Flores, M. A., Contag, C. H., & Kaspar, R. L. (2016). Non-Invasive Intravital Imaging of siRNA-Mediated Mutant Keratin Gene Repression in Skin. Molecular Imaging and Biology, 18(1), 34-42. https://doi.org/10.1007/s11307-015-0875-z