Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

François Bizet, Lionel Xavier Dupuy, Anthony Bengough (Lead / Corresponding author), Alexis Peaucelle, Irène Hummel, Marie-Béatrice Bogeat-Triboulot (Lead / Corresponding author)

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Abstract

Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.
Original languageEnglish
Article numbere2390
Pages (from-to)1-12
Number of pages12
JournalBio-Protocol
DOIs
Publication statusPublished - 20 Jul 2017

Keywords

  • Hydroponics
  • Infrared imaging
  • Kinematics
  • Root growth
  • Time-lapse

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    Bizet, F., Dupuy, L. X., Bengough, A., Peaucelle, A., Hummel, I., & Bogeat-Triboulot, M-B. (2017). Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light. Bio-Protocol, 1-12. [e2390]. https://doi.org/10.21769/BioProtoc.2390