Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

François Bizet; Lionel Xavier Dupuy; Anthony Bengough; Alexis Peaucelle; Irène Hummel; Marie-Béatrice Bogeat-Triboulot

doi:10.21769/BioProtoc.2390

Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

François Bizet, Lionel Xavier Dupuy, Anthony Bengough (Lead / Corresponding author), Alexis Peaucelle, Irène Hummel, Marie-Béatrice Bogeat-Triboulot (Lead / Corresponding author)

Civil Engineering

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Abstract

Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.

Original language	English
Article number	e2390
Pages (from-to)	1-12
Number of pages	12
Journal	Bio-Protocol
DOIs	https://doi.org/10.21769/BioProtoc.2390
Publication status	Published - 20 Jul 2017

Keywords

Hydroponics
Infrared imaging
Kinematics
Root growth
Time-lapse

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10.21769/BioProtoc.2390

Author Accepted Manuscript
The final published version is available at http://dx.doi.org/10.21769/BioProtoc.2390
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Cite this

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title = "Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light",

abstract = "Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.",

keywords = "Hydroponics, Infrared imaging, Kinematics, Root growth, Time-lapse",

author = "Fran{\c c}ois Bizet and Dupuy, {Lionel Xavier} and Anthony Bengough and Alexis Peaucelle and Ir{\`e}ne Hummel and Marie-B{\'e}atrice Bogeat-Triboulot",

note = "FB was supported by a grant from the French National Ministry for Education and Research. The research has received funding from the French National Research Agency through the Laboratory of Excellence ARBRE (ANR-12-LABXARBRE-01) and from Agreenium{\textquoteright}s International Research School. The James Hutton Institute receives funding from the Scottish Government. This protocol was adapted from Bizet et al. (2015) and Bizet et al. (2016).",

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month = jul,

day = "20",

doi = "10.21769/BioProtoc.2390",

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AU - Bizet, François

AU - Dupuy, Lionel Xavier

AU - Bengough, Anthony

AU - Peaucelle, Alexis

AU - Hummel, Irène

AU - Bogeat-Triboulot, Marie-Béatrice

N1 - FB was supported by a grant from the French National Ministry for Education and Research. The research has received funding from the French National Research Agency through the Laboratory of Excellence ARBRE (ANR-12-LABXARBRE-01) and from Agreenium’s International Research School. The James Hutton Institute receives funding from the Scottish Government. This protocol was adapted from Bizet et al. (2015) and Bizet et al. (2016).

PY - 2017/7/20

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N2 - Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.

AB - Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.

KW - Hydroponics

KW - Infrared imaging

KW - Kinematics

KW - Root growth

KW - Time-lapse

U2 - 10.21769/BioProtoc.2390

DO - 10.21769/BioProtoc.2390

M3 - Article

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M1 - e2390

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Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

Abstract

Keywords

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