Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

François Bizet; Lionel Xavier Dupuy; Anthony Bengough; Alexis Peaucelle; Irène Hummel; Marie-Béatrice Bogeat-Triboulot

doi:10.21769/BioProtoc.2390

Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

François Bizet
, Lionel Xavier Dupuy
, Anthony Bengough (Lead / Corresponding author)
, Alexis Peaucelle
, Irène Hummel
, Marie-Béatrice Bogeat-Triboulot (Lead / Corresponding author)

Civil Engineering

Research output: Contribution to journal › Protocol › peer-review

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Abstract

Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.

Original language	English
Article number	e2390
Number of pages	12
Journal	Bio-Protocol
Volume	7
Issue number	14
DOIs	https://doi.org/10.21769/BioProtoc.2390
Publication status	Published - 20 Jul 2017

Keywords

Hydroponics
Infrared imaging
Kinematics
Root growth
Time-lapse

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10.21769/BioProtoc.2390

Author Accepted Manuscript
The final published version is available at http://dx.doi.org/10.21769/BioProtoc.2390
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title = "Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light",

abstract = "Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.",

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AU - Bizet, François

AU - Dupuy, Lionel Xavier

AU - Bengough, Anthony

AU - Peaucelle, Alexis

AU - Hummel, Irène

AU - Bogeat-Triboulot, Marie-Béatrice

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AB - Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for kinematic analyses. It allows continuous imaging at fine spatiotemporal resolution and disturbs roots minimally. Examples are provided of how the procedure and materials can be adapted for 3D monitoring and of how environmental stress may be manipulated for experimental purposes.

KW - Hydroponics

KW - Infrared imaging

KW - Kinematics

KW - Root growth

KW - Time-lapse

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Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

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Keywords

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