Non-targeted metabolite profiling highlights the potential of strawberry leaves as a resource for specific bioactive compounds

Anna Kårlund (Lead / Corresponding author), Kati Hanhineva, Marko Lehtonen, Gordon J. McDougall, Derek Stewart, Reijo O. Karjalainen

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)
422 Downloads (Pure)


Background: The non-edible parts of horticultural crops, such as leaves, contain substantial amounts of valuable bioactive compounds which are currently only little exploited. For example strawberry (Fragaria × ananassa) leaves may be a promising bioresource for diverse health-related applications. However, product standardization sets a real challenge, especially when the leaf material comes from varying cultivars. The first step towards better quality control of berry fruit leaf-based ingredients and supplements is to understand metabolites present and their stability in different plant cultivars, so we surveyed the distribution of potentially bioactive strawberry leaf metabolites in six different strawberry cultivars. Non-targeted metabolite profiling analysis using LC-qTOF-ESI-MS with data processing via principal component analysis and k-means clustering analysis were utilized to examine the differences and commonalities between the leaf metabolite profiles.

Results: Quercetin and kaempferol derivatives were the dominant flavonol groups in strawberry leaves. Previously described and novel caffeic and chlorogenic acid derivatives were among the major phenolic acids. In addition, ellagitannins were one of the distinguishing compound classes in strawberry leaves. In general, strawberry leaves also contained high levels of octadecatrienoic acid derivatives, precursors of valuable odor compounds.

Conclusions: The specific bioactive compounds found in the leaves of different strawberry cultivars offer the potential for the selection of optimized leaf materials for added-value food and for non-food applications.

Original languageEnglish
Pages (from-to)2182-2190
Number of pages9
JournalJournal of the Science of Food and Agriculture
Issue number7
Early online date9 Sept 2016
Publication statusPublished - 3 Apr 2017


  • Fragaria × ananassa
  • leaves
  • non-targeted metabolite profiling
  • polyphenols
  • cultivars


Dive into the research topics of 'Non-targeted metabolite profiling highlights the potential of strawberry leaves as a resource for specific bioactive compounds'. Together they form a unique fingerprint.

Cite this