Projects per year
Abstract
Nonsense-mediated RNA decay (NMD) is an RNA control mechanism that has also been implicated in the broader regulation of gene expression. Nevertheless, a role for NMD in genome regulation has not yet been fully assessed, partially because NMD inactivation is lethal in many organisms. Here, we performed an in-depth comparative analysis of Arabidopsis (Arabidopsis thaliana) mutants lacking the NMD-related proteins UPF3, UPF1, and SMG7. We found different impacts of these proteins on NMD and the Arabidopsis transcriptome, with UPF1 having the biggest effect. Transcriptome assembly in UPF1-null plants revealed genome-wide changes in alternative splicing, suggesting that UPF1 functions in splicing. The inactivation of UPF1 led to translational repression, as manifested by a global shift in mRNAs from polysomes to monosomes and the downregulation of genes involved in translation and ribosome biogenesis. Despite these global changes, NMD targets and mRNAs expressed at low levels with short half-lives were enriched in the polysomes of upf1 mutants, indicating that UPF1/NMD suppresses the translation of aberrant RNAs. Particularly striking was an increase in the translation of TIR domain-containing, nucleotide binding, leucine-rich repeat (TNL) immune receptors. The regulation of TNLs via UPF1/NMD-mediated mRNA stability and translational derepression offers a dynamic mechanism for the rapid activation of TNLs in response to pathogen attack.
Original language | English |
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Pages (from-to) | 2725-2741 |
Number of pages | 17 |
Journal | The Plant Cell |
Volume | 32 |
Issue number | 9 |
Early online date | 14 Jul 2020 |
DOIs | |
Publication status | Published - 4 Sept 2020 |
Keywords
- nonsense mediated RNA decay
- translation
- RNA stability
- RNA splicing
- plant defense
- effector triggered immunity
ASJC Scopus subject areas
- Plant Science
- Cell Biology
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Dive into the research topics of 'Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis'. Together they form a unique fingerprint.Projects
- 1 Finished
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Dynamic Re-programming of the Cold Transcriptome in Arabidopsis (Joint with JHI)
Brown, J. (Investigator)
Biotechnology and Biological Sciences Research Council
1/04/17 → 31/07/20
Project: Research
Student theses
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Automated generation of high quality transcriptome annotations and their coding characterisation
Entizne, J. C. (Author), Brown, J. (Supervisor) & Zhang, R. (Supervisor), 2021Student thesis: Doctoral Thesis › Doctor of Philosophy