Novel colon-available triterpenoids identified in raspberry fruits exhibit antigenotoxic activities in vitro

Gordon J. McDougall (Lead / Corresponding author), J. William Allwood, Gema Pereira-Caro, Emma M. Brown, Susan Verrall, Derek Stewart, Cheryl Latimer, Geoff McMullan, Roger Lawther, Gloria O'Connor, Ian Rowland, Alan Crozier, Chris I. R. Gill

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
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Abstract

SCOPE: Ileostomy studies provide a unique insight into digestion of food, allowing identification of physiologically relevant dietary phytochemicals and their metabolites important to gut health. We previously reported the consistent increase of components in ileal fluids of ileostomates after consumption of raspberries with use of non-targeted LC-MS(n) techniques and data deconvolution software highlighting two major unknown components (m/z 355 and 679).

METHODS AND RESULTS: In-depth LC-MS(n) analyses suggested that the ileal m/z 355 components were p-coumaroyl glucarates. These compounds have not been identified previously and were confirmed in raspberry extracts after partial purification. The major ileal component with m/z 679 was a glycoside with an aglycone of m/z 517 and was present as two peaks in extracts of whole puree, unseeded puree and isolated seeds. These components were purified using Sephadex LH20 and C18 SPE units and identified as major, novel raspberry triterpenoid glycosides. This triterpenoid-enriched fraction (100 nM) protected against H2 O2 -induced DNA damage in both colon cancer and normal cell lines and altered expression of cytoprotective genes.

CONCLUSION: The presence of these novel raspberry triterpenoid components in ileal fluids indicates that they would be colon-available in vivo, so confirmation of their anti-cancer bioactivities is of key physiological relevance. This article is protected by copyright. All rights reserved.

Original languageEnglish
Article number1600327
Number of pages14
JournalMolecular Nutrition & Food Research
Volume61
Issue number2
Early online date10 Sep 2016
DOIs
Publication statusPublished - 3 Feb 2017

Keywords

  • Raspberries
  • metabolic profiling
  • triterpenoids
  • colon cancer
  • ileal fluid

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