Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

A. Mehlert, L. Varon, I. Silman, S. W. Homans, M. A. Ferguson

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
    Original languageEnglish
    Pages (from-to)243-248
    Number of pages6
    JournalBrazilian Journal of Medical and Biological Research
    Volume27
    Issue number2
    Publication statusPublished - 1994

    Fingerprint

    Electric Organ
    Torpedo
    Glycosylphosphatidylinositols
    Inositol
    Acetylcholinesterase
    Anchors
    Membranes
    Ethanolamine
    Methylation
    Polysaccharides
    Magnetic Resonance Spectroscopy
    Nuclear magnetic resonance
    Glucose
    Molecules
    mannosyl alpha(1-6)-mannoside

    Cite this

    @article{e2e2a58bf7454394816fdef8f881eae7,
    title = "Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica",
    abstract = "The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.",
    author = "A. Mehlert and L. Varon and I. Silman and Homans, {S. W.} and Ferguson, {M. A.}",
    note = "Medline is the source for the citation and abstract of this record.",
    year = "1994",
    language = "English",
    volume = "27",
    pages = "243--248",
    journal = "Brazilian Journal of Medical and Biological Research",
    issn = "0100-879X",
    publisher = "Associacao Brasileira de Divulgacao Cientifica",
    number = "2",

    }

    Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica. / Mehlert, A.; Varon, L.; Silman, I.; Homans, S. W.; Ferguson, M. A.

    In: Brazilian Journal of Medical and Biological Research, Vol. 27, No. 2, 1994, p. 243-248.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

    AU - Mehlert, A.

    AU - Varon, L.

    AU - Silman, I.

    AU - Homans, S. W.

    AU - Ferguson, M. A.

    N1 - Medline is the source for the citation and abstract of this record.

    PY - 1994

    Y1 - 1994

    N2 - The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

    AB - The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

    UR - http://www.scopus.com/inward/record.url?scp=0028376213&partnerID=8YFLogxK

    M3 - Article

    VL - 27

    SP - 243

    EP - 248

    JO - Brazilian Journal of Medical and Biological Research

    JF - Brazilian Journal of Medical and Biological Research

    SN - 0100-879X

    IS - 2

    ER -