Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

A. Mehlert, L. Varon, I. Silman, S. W. Homans, M. A. Ferguson

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    Abstract

    The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
    Original languageEnglish
    Pages (from-to)243-248
    Number of pages6
    JournalBrazilian Journal of Medical and Biological Research
    Volume27
    Issue number2
    Publication statusPublished - 1994

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