Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

A. Mehlert; L. Varon; I. Silman; S. W. Homans; M. A. Ferguson

Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

A. Mehlert, L. Varon, I. Silman, S. W. Homans, M. A. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Brazilian Journal of Medical and Biological Research
Volume	27
Issue number	2
Publication status	Published - 1994

Cite this

@article{e2e2a58bf7454394816fdef8f881eae7,

title = "Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica",

abstract = "The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.",

author = "A. Mehlert and L. Varon and I. Silman and Homans, {S. W.} and Ferguson, {M. A.}",

note = "Medline is the source for the citation and abstract of this record.",

year = "1994",

language = "English",

volume = "27",

pages = "243--248",

journal = "Brazilian Journal of Medical and Biological Research",

issn = "0100-879X",

publisher = "Associacao Brasileira de Divulgacao Cientifica",

number = "2",

}

TY - JOUR

T1 - Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

AU - Mehlert, A.

AU - Varon, L.

AU - Silman, I.

AU - Homans, S. W.

AU - Ferguson, M. A.

N1 - Medline is the source for the citation and abstract of this record.

PY - 1994

Y1 - 1994

N2 - The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

AB - The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.

UR - http://www.scopus.com/inward/record.url?scp=0028376213&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028376213

SN - 0100-879X

VL - 27

SP - 243

EP - 248

JO - Brazilian Journal of Medical and Biological Research

JF - Brazilian Journal of Medical and Biological Research

IS - 2

ER -

Novel GPI structures of the membrane anchor of acetylcholinesterase from the electric organ of Torpedo californica

Abstract

Other files and links

Fingerprint

Cite this