Novel GREM1 Variations in Sub-Saharan African Patients With Cleft Lip and/or Cleft Palate

Lord Jephthah Joojo Gowans, Ganiyu Oseni, Peter A. Mossey, Wasiu Lanre Adeyemo, Mekonen A. Eshete, Tamara D. Busch, Peter Donkor, Solomon Obiri-Yeboah, Gyikua Plange-Rhule, Alexander A. Oti, Arwa Owais, Peter B. Olaitan, Babatunde S. Aregbesola, Fadekemi Oginni, Seidu A. Bello, Rosemary Audu, Chika Onwuamah, Pius Agbenorku, Mobolanle O. Ogunlewe, Lukman O. Abdur-RahmanMary L. Marazita, A. A. Adeyemo, Jeffrey C. Murray, Azeez Butali

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Abstract

Objective: Cleft lip and/or cleft palate (CL/P) are congenital anomalies of the face and have multifactorial etiology, with both environmental and genetic risk factors playing crucial roles. Though at least 40 loci have attained genomewide significant association with nonsyndromic CL/P, these loci largely reside in noncoding regions of the human genome, and subsequent resequencing studies of neighboring candidate genes have revealed only a limited number of etiologic coding variants. The present study was conducted to identify etiologic coding variants in GREM1, a locus that has been shown to be largely associated with cleft of both lip and soft palate.

Patients and Method: We resequenced DNA from 397 sub-Saharan Africans with CL/P and 192 controls using Sanger sequencing. Following analyses of the sequence data, we observed 2 novel coding variants in GREM1. These variants were not found in the 192 African controls and have never been previously reported in any public genetic variant database that includes more than 5000 combined African and African American controls or from the CL/P literature.

Results: The novel variants include p.Pro164Ser in an individual with soft palate cleft only and p.Gly61Asp in an individual with bilateral cleft lip and palate. The proband with the p.Gly61Asp GREM1 variant is a van der Woude (VWS) case who also has an etiologic variant in IRF6 gene.

Conclusion: Our study demonstrated that there is low number of etiologic coding variants in GREM1, confirming earlier suggestions that variants in regulatory elements may largely account for the association between this locus and CL/P.

Original languageEnglish
Pages (from-to)736-742
Number of pages7
JournalCleft Palate-Craniofacial Journal
Volume55
Issue number5
Early online date28 Feb 2018
DOIs
Publication statusPublished - 1 May 2018

Keywords

  • Cleft lip and/or cleft palate
  • DNA sequencing
  • GREM1 gene
  • Soft palate cleft
  • Sub-Saharan Africans

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    Mossey, Peter

    • Dentistry - Professor (Clinical) & Personal Chair of Craniofacial Dev & Dentofacial Orthopaeds

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    Cite this

    Gowans, L. J. J., Oseni, G., Mossey, P. A., Adeyemo, W. L., Eshete, M. A., Busch, T. D., Donkor, P., Obiri-Yeboah, S., Plange-Rhule, G., Oti, A. A., Owais, A., Olaitan, P. B., Aregbesola, B. S., Oginni, F., Bello, S. A., Audu, R., Onwuamah, C., Agbenorku, P., Ogunlewe, M. O., ... Butali, A. (2018). Novel GREM1 Variations in Sub-Saharan African Patients With Cleft Lip and/or Cleft Palate. Cleft Palate-Craniofacial Journal, 55(5), 736-742. https://doi.org/10.1177/1055665618754948