NS 1619 activates BKCa channel activity in rat cortical neurones

Kevin Lee, I C Rowe, M L Ashford

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Single channel recordings of large conductance Ca2(+)-activated K+ (BKCa) channels were made from neurones isolated from rat motor cortex. Application of levcromakalim, pinacidil or diazoxide had no effect on BKCa channel activity in excised patches. In contrast, NS 1619 (1-(2'-hydroxy-5'-trifluoromethylphenyl)-5-trifluoromethyl- 2(3H)benzimidazalone) induced concentration-dependent activation of BKCa channels with a calculated EC50 of 32 microM. The NS 1619-induced activity was dependent on the presence of free Ca2+ at the intracellular surface, but was not associated with a change in channel voltage sensitivity. Niflumic acid had no effect on BKCa activity per se but prevented NS 1619-mediated activation.
    Original languageEnglish
    Pages (from-to)215-9
    Number of pages5
    JournalEuropean Journal of Pharmacology
    Volume280
    Issue number2
    DOIs
    Publication statusPublished - 4 Jul 1995

    Keywords

    • Rats
    • Motor Cortex
    • Benzimidazoles
    • Animals
    • Rats, Sprague-Dawley
    • Calcium
    • Neurons
    • Adenosine Triphosphate
    • Potassium Channels
    • Male

    Fingerprint Dive into the research topics of 'NS 1619 activates BKCa channel activity in rat cortical neurones'. Together they form a unique fingerprint.

  • Cite this