TY - JOUR
T1 - Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-β pathways
AU - Alarcón, Claudio
AU - Zaromytidou, Alexia-Ileana
AU - Xi, Qiaoran
AU - Gao, Sheng
AU - Yu, Jianzhong
AU - Fujisawa, Sho
AU - Barlas, Afsar
AU - Miller, Alexandria N.
AU - Manova-Todorova, Katia
AU - Macias, Maria J.
AU - Sapkota, Gopal
AU - Pan, Duojia
AU - Massagué, Joan
PY - 2009/11/13
Y1 - 2009/11/13
N2 - TGF-ß and BMP receptor kinases activate Smad transcription factors by C-terminal phosphorylation. We have identified a subsequent agonist-induced phosphorylation that plays a central dual role in Smad transcriptional activation and turnover. As receptor-activated Smads form transcriptional complexes, they are phosphorylated at an interdomain linker region by CDK8 and CDK9, which are components of transcriptional mediator and elongation complexes. These phosphorylations promote Smad transcriptional action, which in the case of Smad1 is mediated by the recruitment of YAP to the phosphorylated linker sites. An effector of the highly conserved Hippo organ size control pathway, YAP supports Smad1-dependent transcription and is required for BMP suppression of neural differentiation of mouse embryonic stem cells. The phosphorylated linker is ultimately recognized by specific ubiquitin ligases, leading to proteasome-mediated turnover of activated Smad proteins. Thus, nuclear CDK8/9 drive a cycle of Smad utilization and disposal that is an integral part of canonical BMP and TGF-ß pathways.
AB - TGF-ß and BMP receptor kinases activate Smad transcription factors by C-terminal phosphorylation. We have identified a subsequent agonist-induced phosphorylation that plays a central dual role in Smad transcriptional activation and turnover. As receptor-activated Smads form transcriptional complexes, they are phosphorylated at an interdomain linker region by CDK8 and CDK9, which are components of transcriptional mediator and elongation complexes. These phosphorylations promote Smad transcriptional action, which in the case of Smad1 is mediated by the recruitment of YAP to the phosphorylated linker sites. An effector of the highly conserved Hippo organ size control pathway, YAP supports Smad1-dependent transcription and is required for BMP suppression of neural differentiation of mouse embryonic stem cells. The phosphorylated linker is ultimately recognized by specific ubiquitin ligases, leading to proteasome-mediated turnover of activated Smad proteins. Thus, nuclear CDK8/9 drive a cycle of Smad utilization and disposal that is an integral part of canonical BMP and TGF-ß pathways.
U2 - 10.1016/j.cell.2009.09.035
DO - 10.1016/j.cell.2009.09.035
M3 - Article
C2 - 19914168
SN - 1097-4172
VL - 139
SP - 757
EP - 769
JO - Cell
JF - Cell
IS - 4
ER -