Nuclear functions in space and time: Gene expression in a dynamic, constrained environment

Laura Trinkle-Mulcahy, Angus I. Lamond

    Research output: Contribution to journalReview article

    22 Citations (Scopus)

    Abstract

    All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanisms remain to be answered, including the relationship between the spatial organization of the genome and the transcription machinery. Powerful in vivo approaches are allowing researchers to test established in vitro concepts within the dynamic cellular environment, while genome-wide screens have enabled rapid high throughput analyses of both structural and functional parameters. In several cases, as highlighted here, this has turned up surprising results and has forced a re-evaluation of models for nuclear structure and gene regulation. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)1960-1970
    Number of pages11
    JournalFEBS Letters
    Volume582
    Issue number14
    DOIs
    Publication statusPublished - 18 Jun 2008

    Keywords

    • nucleus
    • transcription
    • chromatin
    • dynamic
    • organization
    • RNA-POLYMERASE-II
    • LIVING CELLS
    • REAL-TIME
    • IN-VIVO
    • CHROMOSOME TERRITORIES
    • TRANSCRIPTIONAL REGULATION
    • GENOME ORGANIZATION
    • INTERPHASE NUCLEUS
    • CHROMATIN DOMAINS
    • DNA INTERACTIONS

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