Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies

Anthony H. Stonehouse; Blair D. Grubb; J. Howard Pringle; Robert I. Norman; Peter R. Stanfield; William J. Brammar

doi:10.1385/JMN:20:2:189

Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies

Anthony H. Stonehouse, Blair D. Grubb, J. Howard Pringle, Robert I. Norman, Peter R. Stanfield, William J. Brammar

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

Original language	English
Pages (from-to)	189-193
Number of pages	5
Journal	Journal of Molecular Neuroscience
Volume	20
Issue number	2
DOIs	https://doi.org/10.1385/JMN:20:2:189
Publication status	Published - May 2003

Keywords

Affinity-purified polyclonal antibody
Dorsal root ganglia
Fastigial nuclei
Kir2.2
Nuclear staining
Vestibular nuclei

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

Access to Document

10.1385/JMN:20:2:189

Cite this

@article{303d2a1cc7bc4eb29387dfe465beec56,

title = "Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies",

abstract = "The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.",

keywords = "Affinity-purified polyclonal antibody, Dorsal root ganglia, Fastigial nuclei, Kir2.2, Nuclear staining, Vestibular nuclei",

author = "Stonehouse, {Anthony H.} and Grubb, {Blair D.} and Pringle, {J. Howard} and Norman, {Robert I.} and Stanfield, {Peter R.} and Brammar, {William J.}",

year = "2003",

month = may,

doi = "10.1385/JMN:20:2:189",

language = "English",

volume = "20",

pages = "189--193",

journal = "Journal of Molecular Neuroscience",

issn = "0895-8696",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies

AU - Stonehouse, Anthony H.

AU - Grubb, Blair D.

AU - Pringle, J. Howard

AU - Norman, Robert I.

AU - Stanfield, Peter R.

AU - Brammar, William J.

PY - 2003/5

Y1 - 2003/5

N2 - The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

AB - The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

KW - Affinity-purified polyclonal antibody

KW - Dorsal root ganglia

KW - Fastigial nuclei

KW - Kir2.2

KW - Nuclear staining

KW - Vestibular nuclei

UR - http://www.scopus.com/inward/record.url?scp=0038279929&partnerID=8YFLogxK

U2 - 10.1385/JMN:20:2:189

DO - 10.1385/JMN:20:2:189

M3 - Article

C2 - 12794312

AN - SCOPUS:0038279929

SN - 0895-8696

VL - 20

SP - 189

EP - 193

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

IS - 2

ER -

Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this