Nuclear immunostaining in rat neuronal cells using two anti-Kir2.2 ion channel polyclonal antibodies

Anthony H. Stonehouse, Blair D. Grubb, J. Howard Pringle, Robert I. Norman, Peter R. Stanfield, William J. Brammar

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    7 Citations (Scopus)

    Abstract

    The inwardly rectifying potassium ion channel Kir2.2 has recently been demonstrated to have nuclear and plasma membrane subcellular localization. Nuclear expression of Kir2.2 is controversial, as a functional role for Kir2.0 potassium channels in the nucleus has not been investigated. However, in this report we have demonstrated Kir2.2 nuclear localization in sections of rat hindbrain and dorsal root ganglia tissue, using two anti-Kir2.2 polyclonal antisera with different epitope specificities. These data confirm nuclear localization and are suggestive of new functions of Kir2.0 potassium ion channels in the nucleus.

    Original languageEnglish
    Pages (from-to)189-193
    Number of pages5
    JournalJournal of Molecular Neuroscience
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - May 2003

    Keywords

    • Affinity-purified polyclonal antibody
    • Dorsal root ganglia
    • Fastigial nuclei
    • Kir2.2
    • Nuclear staining
    • Vestibular nuclei

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