Nuclear Speckles

David L. Spector, Angus I. Lamond

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    386 Citations (Scopus)

    Abstract

    Nuclear speckles, also known as interchromatin granule clusters, are nuclear domains enriched in pre-mRNA splicing factors, located in the interchromatin regions of the nucleoplasm of mammalian cells. When observed by immunofluorescence microscopy, they usually appear as 20-50 irregularly shaped structures that vary in size. Speckles are dynamic structures, and their constituents can exchange continuously with the nucleoplasm and other nuclear locations, including active transcription sites. Studies on the composition, structure, and dynamics of speckles have provided an important paradigm for understanding the functional organization of the nucleus and the dynamics of the gene expression machinery.

    Original languageEnglish
    Article numbera000646
    Pages (from-to)-
    Number of pages12
    JournalCold spring harbor perspectives in biology
    Volume3
    Issue number2
    DOIs
    Publication statusPublished - Feb 2011

    Keywords

    • RNA-POLYMERASE-II
    • INTERCHROMATIN GRANULE CLUSTERS
    • MAMMALIAN-CELL NUCLEUS
    • SPLICING FACTORS
    • SC-35 DOMAINS
    • SERINE-RICH
    • IN-VIVO
    • SUBNUCLEAR LOCALIZATION
    • PROTEOMIC ANALYSIS
    • POLY(A) RNA

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