Nucleobase participation in ribozyme catalysis

Zheng-yun Zhao, Aileen McLeod, Shinya Harusawa, Lisa Araki, Maho Yamaguchi, Takushi Kurihara, David M. J. Lilley

    Research output: Contribution to journalArticlepeer-review

    54 Citations (Scopus)

    Abstract

    We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are consistent with a role for nucleobase catalysis in the catalytic mechanism of this ribozyme.
    Original languageEnglish
    Pages (from-to)5026-5027
    Number of pages2
    JournalJournal of the American Chemical Society
    Volume127
    Issue number14
    DOIs
    Publication statusPublished - 2005

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