Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?

David W. Meek, Richard S. Hayward

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    The “a-operon” of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (In order of transcription, and with their products named in brackets): rpsm (S13), rpsK (S11), rpsD (S4), rpoA (a-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the‘rpsD–rpoA’ sequences previously determined by others, to include allrpoA and rplQ. The rpoA–rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.
    Original languageEnglish
    Pages (from-to)5813-5821
    Number of pages9
    JournalNucleic Acids Research
    Volume12
    Issue number14
    DOIs
    Publication statusPublished - 25 Jul 1984

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    5-chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide
    DNA-Directed RNA Polymerases
    Carrier Proteins
    Binding Sites
    Escherichia coli
    DNA
    Genes
    Intergenic DNA
    Ribosomal Proteins
    Protein Biosynthesis
    Operon
    Messenger RNA

    Cite this

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    title = "Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?",
    abstract = "The “a-operon” of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (In order of transcription, and with their products named in brackets): rpsm (S13), rpsK (S11), rpsD (S4), rpoA (a-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the‘rpsD–rpoA’ sequences previously determined by others, to include allrpoA and rplQ. The rpoA–rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.",
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    Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli : a second regulatory binding site for protein S4? / Meek, David W.; Hayward, Richard S.

    In: Nucleic Acids Research, Vol. 12, No. 14, 25.07.1984, p. 5813-5821.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli

    T2 - a second regulatory binding site for protein S4?

    AU - Meek, David W.

    AU - Hayward, Richard S.

    PY - 1984/7/25

    Y1 - 1984/7/25

    N2 - The “a-operon” of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (In order of transcription, and with their products named in brackets): rpsm (S13), rpsK (S11), rpsD (S4), rpoA (a-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the‘rpsD–rpoA’ sequences previously determined by others, to include allrpoA and rplQ. The rpoA–rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.

    AB - The “a-operon” of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (In order of transcription, and with their products named in brackets): rpsm (S13), rpsK (S11), rpsD (S4), rpoA (a-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the‘rpsD–rpoA’ sequences previously determined by others, to include allrpoA and rplQ. The rpoA–rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.

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