Omics of Food-Borne Gastroenteritis: Global Proteomic and Mutagenic Analysis of Salmonella enterica Serovar Enteritidis

Aryashree Arunima, Soujanya D. Yelamanchi, Chandrashekhar Padhi, Sangeeta Jaiswal, Daniel Ryan, Bhawna Gupta, Gajanan Sathe, Jayshree Advani, Harsha Gowda, T. S. Keshava Prasad, Mrutyunjay Suar (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Salmonella Enteritidis causes food-borne gastroenteritis by the two type three secretion systems (TTSS). TTSS-1 mediates invasion through intestinal lining, and TTSS-2 facilitates phagocytic survival. The pathogens' ability to infect effectively under TTSS-1-deficient background in host's phagocytes is poorly understood. Therefore, pathobiological understanding of TTSS-1-defective nontyphoidal Salmonellosis is highly important. We performed a comparative global proteomic analysis of the isogenic TTSS-1 mutant of Salmonella Enteritidis (M1511) and its wild-type isolate P125109. Our results showed 43 proteins were differentially expressed. Functional annotation further revealed that differentially expressed proteins belong to pathogenesis, tRNA and ncRNA metabolic processes. Three proteins, tryptophan subunit alpha chain, citrate lyase subunit alpha, and hypothetical protein 3202, were selected for in vitro analysis based on their functional annotations. Deletion mutants generated for the above proteins in the M1511 strain showed reduced intracellular survival inside macrophages in vitro. In sum, this study provides mass spectrometry-based evidence for seven hypothetical proteins, which will be subject of future investigations. Our study identifies proteins influencing virulence of Salmonella in the host. The study complements and further strengthens previously published research on proteins involved in enteropathogenesis of Salmonella and extends their role in noninvasive Salmonellosis.

Original languageEnglish
Pages (from-to)571-583
Number of pages13
JournalOMICS A Journal of Integrative Biology
Volume21
Issue number10
Early online date1 Oct 2017
DOIs
Publication statusPublished - Oct 2017

Keywords

  • enteric pathogen
  • Food-borne gastroenteritis
  • LTQ-Orbitrap Velos mass spectrometer
  • membrane proteins
  • nutritional immunity

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