On-Chip Screening of a Glycomimetic Library with C-Type Lectins Reveals Structural Features Responsible for Preferential Binding of Dectin-2 over DC-SIGN/R and Langerin

Laura Medve, Silvia Achilli, Sonia Serna, Fabio Zuccotto, Norbert Varga, Michel Thépaut, Monica Civera, Corinne Vivès, Franck Fieschi, Niels Reichardt, Anna Bernardi

Research output: Contribution to journalArticle

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Abstract

A library of mannose- and fucose-based glycomimetics was synthesized and screened in a microarray format against a set of C-type lectin receptors (CLRs) that included DC-SIGN, DC-SIGNR, langerin, and dectin-2. Glycomimetic ligands able to interact with dectin-2 were identified for the first time. Comparative analysis of binding profiles allowed their selectivity against other CLRs to be probed.

Original languageEnglish
Pages (from-to)14448-14460
Number of pages13
JournalChemistry - A European Journal
Volume24
Issue number54
Early online date5 Jul 2018
DOIs
Publication statusPublished - 25 Sep 2018

Fingerprint

C-Type Lectins
Screening
Fucose
Microarrays
Mannose
Ligands
DC-specific ICAM-3 grabbing nonintegrin
mouse dectin-2
Lectins

Keywords

  • C-type lectins
  • carbohydrates
  • drug discovery
  • glycomimetics
  • microarrays

Cite this

Medve, Laura ; Achilli, Silvia ; Serna, Sonia ; Zuccotto, Fabio ; Varga, Norbert ; Thépaut, Michel ; Civera, Monica ; Vivès, Corinne ; Fieschi, Franck ; Reichardt, Niels ; Bernardi, Anna. / On-Chip Screening of a Glycomimetic Library with C-Type Lectins Reveals Structural Features Responsible for Preferential Binding of Dectin-2 over DC-SIGN/R and Langerin. In: Chemistry - A European Journal. 2018 ; Vol. 24, No. 54. pp. 14448-14460.
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abstract = "A library of mannose- and fucose-based glycomimetics was synthesized and screened in a microarray format against a set of C-type lectin receptors (CLRs) that included DC-SIGN, DC-SIGNR, langerin, and dectin-2. Glycomimetic ligands able to interact with dectin-2 were identified for the first time. Comparative analysis of binding profiles allowed their selectivity against other CLRs to be probed.",
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On-Chip Screening of a Glycomimetic Library with C-Type Lectins Reveals Structural Features Responsible for Preferential Binding of Dectin-2 over DC-SIGN/R and Langerin. / Medve, Laura; Achilli, Silvia; Serna, Sonia; Zuccotto, Fabio; Varga, Norbert; Thépaut, Michel; Civera, Monica; Vivès, Corinne; Fieschi, Franck; Reichardt, Niels; Bernardi, Anna.

In: Chemistry - A European Journal, Vol. 24, No. 54, 25.09.2018, p. 14448-14460.

Research output: Contribution to journalArticle

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AU - Medve, Laura

AU - Achilli, Silvia

AU - Serna, Sonia

AU - Zuccotto, Fabio

AU - Varga, Norbert

AU - Thépaut, Michel

AU - Civera, Monica

AU - Vivès, Corinne

AU - Fieschi, Franck

AU - Reichardt, Niels

AU - Bernardi, Anna

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