One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Kety Giannetti, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Efthymios Kyrodimos, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios ValakosGiannis Vatsellas, Russell Petty, Dimitris Thanos, Marco Demaria, Konstantinos Evangelou, Raffaella Di Micco, Vassilis G Gorgoulis (Lead / Corresponding author)

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Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.

Original languageEnglish
Article number102929
Number of pages16
JournalSTAR Protocols
Issue number1
Early online date8 Mar 2024
Publication statusPublished - 15 Mar 2024


  • Animals
  • Cell Separation
  • Flow Cytometry
  • Fluorescent Dyes
  • Models, Animal
  • Cellular Senescence
  • Cell-based Assays
  • Molecular/Chemical Probes
  • Cell isolation
  • Cancer
  • Cell Biology

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology
  • General Neuroscience


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