TY - JOUR
T1 - One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16
AU - Magkouta, Sophia
AU - Veroutis, Dimitris
AU - Pousias, Athanasios
AU - Papaspyropoulos, Angelos
AU - Giannetti, Kety
AU - Pippa, Natassa
AU - Lougiakis, Nikolaos
AU - Kambas, Konstantinos
AU - Lagopati, Nefeli
AU - Polyzou, Aikaterini
AU - Georgiou, Maria
AU - Chountoulesi, Maria
AU - Pispas, Stergios
AU - Foutadakis, Spyros
AU - Kyrodimos, Efthymios
AU - Pouli, Nicole
AU - Marakos, Panagiotis
AU - Kotsinas, Athanassios
AU - Verginis, Panayotis
AU - Valakos, Dimitrios
AU - Vatsellas, Giannis
AU - Petty, Russell
AU - Thanos, Dimitris
AU - Demaria, Marco
AU - Evangelou, Konstantinos
AU - Di Micco, Raffaella
AU - Gorgoulis, Vassilis G
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/3/15
Y1 - 2024/3/15
N2 - Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.
AB - Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.
KW - Animals
KW - Cell Separation
KW - Flow Cytometry
KW - Fluorescent Dyes
KW - Models, Animal
KW - Cellular Senescence
KW - Cell-based Assays
KW - Molecular/Chemical Probes
KW - Cell isolation
KW - Cancer
KW - Cell Biology
UR - http://www.scopus.com/inward/record.url?scp=85187001165&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2024.102929
DO - 10.1016/j.xpro.2024.102929
M3 - Article
C2 - 38460134
SN - 2666-1667
VL - 5
JO - STAR Protocols
JF - STAR Protocols
IS - 1
M1 - 102929
ER -