One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Sophia Magkouta; Dimitris Veroutis; Athanasios Pousias; Angelos Papaspyropoulos; Kety Giannetti; Natassa Pippa; Nikolaos Lougiakis; Konstantinos Kambas; Nefeli Lagopati; Aikaterini Polyzou; Maria Georgiou; Maria Chountoulesi; Stergios Pispas; Spyros Foutadakis; Efthymios Kyrodimos; Nicole Pouli; Panagiotis Marakos; Athanassios Kotsinas; Panayotis Verginis; Dimitrios Valakos; Giannis Vatsellas; Russell Petty; Dimitris Thanos; Marco Demaria; Konstantinos Evangelou; Raffaella Di Micco; Vassilis G. Gorgoulis

doi:10.1016/j.xpro.2024.102929

One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Sophia Magkouta
, Dimitris Veroutis
, Athanasios Pousias
, Angelos Papaspyropoulos
, Kety Giannetti
, Natassa Pippa
, Nikolaos Lougiakis
, Konstantinos Kambas
, Nefeli Lagopati
, Aikaterini Polyzou
, Maria Georgiou
, Maria Chountoulesi
, Stergios Pispas
, Spyros Foutadakis
, Efthymios Kyrodimos
, Nicole Pouli
, Panagiotis Marakos
, Athanassios Kotsinas
, Panayotis Verginis
, Dimitrios Valakos

Giannis Vatsellas, Russell Petty, Dimitris Thanos, Marco Demaria, Konstantinos Evangelou, Raffaella Di Micco, Vassilis G. Gorgoulis (Lead / Corresponding author)

Cancer Research

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

155 Downloads (Pure)

Abstract

Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.

Original language	English
Article number	102929
Number of pages	16
Journal	STAR Protocols
Volume	5
Issue number	1
Early online date	8 Mar 2024
DOIs	https://doi.org/10.1016/j.xpro.2024.102929
Publication status	Published - 15 Mar 2024

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Cell Separation
Flow Cytometry
Fluorescent Dyes
Models, Animal
Cellular Senescence
Cell-based Assays
Molecular/Chemical Probes
Cell isolation
Cancer
Cell Biology

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology
General Neuroscience

Access to Document

10.1016/j.xpro.2024.102929Licence: CC BY-NC-ND

Final published versionFinal published version, 4.98 MBLicence: CC BY-NC-ND

Cite this

Magkouta, S., Veroutis, D., Pousias, A., Papaspyropoulos, A., Giannetti, K., Pippa, N., Lougiakis, N., Kambas, K., Lagopati, N., Polyzou, A., Georgiou, M., Chountoulesi, M., Pispas, S., Foutadakis, S., Kyrodimos, E., Pouli, N., Marakos, P., Kotsinas, A., Verginis, P., ... Gorgoulis, V. G. (2024). One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16. STAR Protocols, 5(1), Article 102929. https://doi.org/10.1016/j.xpro.2024.102929

@article{d0c621758498417b92e0e85c5945adbe,

title = "One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16",

abstract = "Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.",

keywords = "Animals, Cell Separation, Flow Cytometry, Fluorescent Dyes, Models, Animal, Cellular Senescence, Cell-based Assays, Molecular/Chemical Probes, Cell isolation, Cancer, Cell Biology",

author = "Sophia Magkouta and Dimitris Veroutis and Athanasios Pousias and Angelos Papaspyropoulos and Kety Giannetti and Natassa Pippa and Nikolaos Lougiakis and Konstantinos Kambas and Nefeli Lagopati and Aikaterini Polyzou and Maria Georgiou and Maria Chountoulesi and Stergios Pispas and Spyros Foutadakis and Efthymios Kyrodimos and Nicole Pouli and Panagiotis Marakos and Athanassios Kotsinas and Panayotis Verginis and Dimitrios Valakos and Giannis Vatsellas and Russell Petty and Dimitris Thanos and Marco Demaria and Konstantinos Evangelou and \{Di Micco\}, Raffaella and Gorgoulis, \{Vassilis G.\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = mar,

day = "15",

doi = "10.1016/j.xpro.2024.102929",

language = "English",

volume = "5",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

Magkouta, S, Veroutis, D, Pousias, A, Papaspyropoulos, A, Giannetti, K, Pippa, N, Lougiakis, N, Kambas, K, Lagopati, N, Polyzou, A, Georgiou, M, Chountoulesi, M, Pispas, S, Foutadakis, S, Kyrodimos, E, Pouli, N, Marakos, P, Kotsinas, A, Verginis, P, Valakos, D, Vatsellas, G, Petty, R, Thanos, D, Demaria, M, Evangelou, K, Di Micco, R & Gorgoulis, VG 2024, 'One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16', STAR Protocols, vol. 5, no. 1, 102929. https://doi.org/10.1016/j.xpro.2024.102929

TY - JOUR

T1 - One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

AU - Magkouta, Sophia

AU - Veroutis, Dimitris

AU - Pousias, Athanasios

AU - Papaspyropoulos, Angelos

AU - Giannetti, Kety

AU - Pippa, Natassa

AU - Lougiakis, Nikolaos

AU - Kambas, Konstantinos

AU - Lagopati, Nefeli

AU - Polyzou, Aikaterini

AU - Georgiou, Maria

AU - Chountoulesi, Maria

AU - Pispas, Stergios

AU - Foutadakis, Spyros

AU - Kyrodimos, Efthymios

AU - Pouli, Nicole

AU - Marakos, Panagiotis

AU - Kotsinas, Athanassios

AU - Verginis, Panayotis

AU - Valakos, Dimitrios

AU - Vatsellas, Giannis

AU - Petty, Russell

AU - Thanos, Dimitris

AU - Demaria, Marco

AU - Evangelou, Konstantinos

AU - Di Micco, Raffaella

AU - Gorgoulis, Vassilis G.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.

AB - Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.

KW - Animals

KW - Cell Separation

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Models, Animal

KW - Cellular Senescence

KW - Cell-based Assays

KW - Molecular/Chemical Probes

KW - Cell isolation

KW - Cancer

KW - Cell Biology

UR - https://www.scopus.com/pages/publications/85187001165

U2 - 10.1016/j.xpro.2024.102929

DO - 10.1016/j.xpro.2024.102929

M3 - Article

C2 - 38460134

SN - 2666-1667

VL - 5

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102929

ER -

One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this