Optically enhanced acoustophoresis

Craig McDougall; Paul O'Mahoney; Alan McGuinn; Nicholas A. Willoughby; Yongqiang  Qiu; Christine E. M. Démoré; Michael MacDonald

doi:10.1117/12.2276323

Optically enhanced acoustophoresis

Craig McDougall, Paul O'Mahoney, Alan McGuinn, Nicholas A. Willoughby, Yongqiang Qiu, Christine E. M. Démoré, Michael MacDonald

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

213 Downloads (Pure)

Abstract

Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting

Original language	English
Title of host publication	Proceedings of SPIE
Subtitle of host publication	Optical Trapping and Optical Micromanipulation XIV
Editors	Kishan Dholakia, Gabriel C. Spalding
Place of Publication	United States of America
Publisher	SPIE-International Society for Optical Engineering
Pages	1-10
Number of pages	10
Volume	10347
ISBN (Electronic)	9781510611528
ISBN (Print)	9781510611511
DOIs	https://doi.org/10.1117/12.2276323
Publication status	Published - 25 Aug 2017
Event	SPIE NanoScience + Engineering: Optical Trapping and Optical Micromanipulation XIV - San Diego, United States Duration: 6 Aug 2017 → 10 Aug 2017

Publication series

Name	Proceedings of SPIE
Publisher	SPIE - International Society for Optical Engineering
Volume	10347
ISSN (Print)	0277-786X
ISSN (Electronic)	1995-756X

Conference

Conference	SPIE NanoScience + Engineering
Country/Territory	United States
City	San Diego
Period	6/08/17 → 10/08/17

Keywords

Cell sorting
acoustrophoresis
optical trapping
optical guiding
cell therapy
bioprocessing

Access to Document

10.1117/12.2276323

Author Accepted Manuscript
Copyright 2017 Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited.
Accepted author manuscript, 610 KB

Scale Up of Optical Fractionation for Bio-Processing (Joint with Heriot Watt University)
MacDonald, M. (Investigator)
Engineering and Physical Sciences Research Council
1/05/14 → 31/12/15
Project: Research

Cite this

McDougall, C., O'Mahoney, P., McGuinn, A., Willoughby, N. A., Qiu, Y., Démoré, C. E. M., & MacDonald, M. (2017). Optically enhanced acoustophoresis. In K. Dholakia, & G. C. Spalding (Eds.), Proceedings of SPIE: Optical Trapping and Optical Micromanipulation XIV (Vol. 10347, pp. 1-10). Article 103471B (Proceedings of SPIE; Vol. 10347). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2276323

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McDougall, C, O'Mahoney, P, McGuinn, A, Willoughby, NA, Qiu, Y, Démoré, CEM & MacDonald, M 2017, Optically enhanced acoustophoresis. in K Dholakia & GC Spalding (eds), Proceedings of SPIE: Optical Trapping and Optical Micromanipulation XIV. vol. 10347, 103471B, Proceedings of SPIE, vol. 10347, SPIE-International Society for Optical Engineering, United States of America, pp. 1-10, SPIE NanoScience + Engineering, San Diego, California, United States, 6/08/17. https://doi.org/10.1117/12.2276323

Optically enhanced acoustophoresis. / McDougall, Craig; O'Mahoney, Paul; McGuinn, Alan et al.
Proceedings of SPIE: Optical Trapping and Optical Micromanipulation XIV. ed. / Kishan Dholakia; Gabriel C. Spalding. Vol. 10347 United States of America: SPIE-International Society for Optical Engineering, 2017. p. 1-10 103471B (Proceedings of SPIE; Vol. 10347).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Optically enhanced acoustophoresis

AU - McDougall, Craig

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AU - McGuinn, Alan

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AU - Qiu, Yongqiang

AU - Démoré, Christine E. M.

AU - MacDonald, Michael

PY - 2017/8/25

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N2 - Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting

AB - Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting

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ER -

McDougall C, O'Mahoney P, McGuinn A, Willoughby NA, Qiu Y, Démoré CEM et al. Optically enhanced acoustophoresis. In Dholakia K, Spalding GC, editors, Proceedings of SPIE: Optical Trapping and Optical Micromanipulation XIV. Vol. 10347. United States of America: SPIE-International Society for Optical Engineering. 2017. p. 1-10. 103471B. (Proceedings of SPIE). doi: 10.1117/12.2276323

Optically enhanced acoustophoresis

Abstract

Publication series

Conference

Keywords

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Projects

Scale Up of Optical Fractionation for Bio-Processing (Joint with Heriot Watt University)

Cite this