Optimal repetition rate and pulse duration studies for two photon imaging

Shamil Mirkhanov, Adrian H. Quarterman, Connor J.C.P. Smyth, Bavishna B. Praveen, Paul Appleton, Calum Thomson, Samuel Swift, Keith G. Wilcox

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Multiphoton imaging (MPI) is an important fluorescence microscopy technique that allows deep tissue and in-vivo imaging with high selectivity. According to theory, two-photon signal is proportional to the product of the peak power and the average power, allowing optimization of key imaging parameters of the excitation laser, such as average power, repetition rate and pulse duration. Recent progress in compact ultrafast lasers including femtosecond fiber lasers and optically pumped semiconductor lasers makes direct control of these parameters possible. In order to investigate the optimum laser parameters for two photon imaging we experimentally study the effects of repetition rate between 2.85 and 90 MHz and pulse duration between 336 fs and 3.5 ps on two photon signal in SYTOX Green labeled mouse intestine sections at 1030 nm. We found that the optimum repetition rate for this sample is in the range 20 - 40 MHz, depending on average power, and that the pulse duration has no effect on the MPI signal provided that the average power can be adjusted to keep the product of average and peak power constant.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVII
PublisherSPIE-International Society for Optical Engineering
Volume10069
ISBN (Electronic)9781510605794
DOIs
Publication statusPublished - 28 Jan 2017
EventSPIE Photonics West 2017: Multiphoton Microscopy in the Biomedical Sciences XVII - Moscone Center, San Francisco, United States
Duration: 29 Jan 201731 Jan 2017
http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2017 (Link to Conference details)

Conference

ConferenceSPIE Photonics West 2017
CountryUnited States
CitySan Francisco
Period29/01/1731/01/17
Internet address

Fingerprint

Photons
repetition
pulse duration
Lasers
Heart Rate
Imaging techniques
photons
Semiconductor Lasers
lasers
Ultrafast lasers
intestines
Fluorescence Microscopy
Laser excitation
Fluorescence microscopy
Intestines
products
Fiber lasers
fiber lasers
mice
Semiconductor lasers

Keywords

  • fiber lasers
  • fluorescence microscopy
  • Multiphoton microscopy
  • ultrafast lasers

Cite this

Mirkhanov, S., Quarterman, A. H., Smyth, C. J. C. P., Praveen, B. B., Appleton, P., Thomson, C., ... Wilcox, K. G. (2017). Optimal repetition rate and pulse duration studies for two photon imaging. In Multiphoton Microscopy in the Biomedical Sciences XVII (Vol. 10069). [100692F] SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2251848
Mirkhanov, Shamil ; Quarterman, Adrian H. ; Smyth, Connor J.C.P. ; Praveen, Bavishna B. ; Appleton, Paul ; Thomson, Calum ; Swift, Samuel ; Wilcox, Keith G. / Optimal repetition rate and pulse duration studies for two photon imaging. Multiphoton Microscopy in the Biomedical Sciences XVII. Vol. 10069 SPIE-International Society for Optical Engineering, 2017.
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Mirkhanov, S, Quarterman, AH, Smyth, CJCP, Praveen, BB, Appleton, P, Thomson, C, Swift, S & Wilcox, KG 2017, Optimal repetition rate and pulse duration studies for two photon imaging. in Multiphoton Microscopy in the Biomedical Sciences XVII. vol. 10069, 100692F, SPIE-International Society for Optical Engineering, SPIE Photonics West 2017, San Francisco, United States, 29/01/17. https://doi.org/10.1117/12.2251848

Optimal repetition rate and pulse duration studies for two photon imaging. / Mirkhanov, Shamil; Quarterman, Adrian H.; Smyth, Connor J.C.P.; Praveen, Bavishna B.; Appleton, Paul; Thomson, Calum; Swift, Samuel; Wilcox, Keith G.

Multiphoton Microscopy in the Biomedical Sciences XVII. Vol. 10069 SPIE-International Society for Optical Engineering, 2017. 100692F.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Appleton, Paul

AU - Thomson, Calum

AU - Swift, Samuel

AU - Wilcox, Keith G.

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N2 - Multiphoton imaging (MPI) is an important fluorescence microscopy technique that allows deep tissue and in-vivo imaging with high selectivity. According to theory, two-photon signal is proportional to the product of the peak power and the average power, allowing optimization of key imaging parameters of the excitation laser, such as average power, repetition rate and pulse duration. Recent progress in compact ultrafast lasers including femtosecond fiber lasers and optically pumped semiconductor lasers makes direct control of these parameters possible. In order to investigate the optimum laser parameters for two photon imaging we experimentally study the effects of repetition rate between 2.85 and 90 MHz and pulse duration between 336 fs and 3.5 ps on two photon signal in SYTOX Green labeled mouse intestine sections at 1030 nm. We found that the optimum repetition rate for this sample is in the range 20 - 40 MHz, depending on average power, and that the pulse duration has no effect on the MPI signal provided that the average power can be adjusted to keep the product of average and peak power constant.

AB - Multiphoton imaging (MPI) is an important fluorescence microscopy technique that allows deep tissue and in-vivo imaging with high selectivity. According to theory, two-photon signal is proportional to the product of the peak power and the average power, allowing optimization of key imaging parameters of the excitation laser, such as average power, repetition rate and pulse duration. Recent progress in compact ultrafast lasers including femtosecond fiber lasers and optically pumped semiconductor lasers makes direct control of these parameters possible. In order to investigate the optimum laser parameters for two photon imaging we experimentally study the effects of repetition rate between 2.85 and 90 MHz and pulse duration between 336 fs and 3.5 ps on two photon signal in SYTOX Green labeled mouse intestine sections at 1030 nm. We found that the optimum repetition rate for this sample is in the range 20 - 40 MHz, depending on average power, and that the pulse duration has no effect on the MPI signal provided that the average power can be adjusted to keep the product of average and peak power constant.

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Mirkhanov S, Quarterman AH, Smyth CJCP, Praveen BB, Appleton P, Thomson C et al. Optimal repetition rate and pulse duration studies for two photon imaging. In Multiphoton Microscopy in the Biomedical Sciences XVII. Vol. 10069. SPIE-International Society for Optical Engineering. 2017. 100692F https://doi.org/10.1117/12.2251848