Organisation and expression of a potato (Solanum tuberosum) protein kinase gene

Rhonda C. Meyer (Lead / Corresponding author), Peter E. Hedley, Robbie Waugh, Gordon C. Machray

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    Abstract

    A 4.3 kb genomic clone from potato (Solanum tuberosum) cv. Saturna has been isolated which contains the full coding sequence of a protein kinase gene (Stpk1), 1 kb of 3' sequence, and the first exon of a neighbouring gene encoding a sucrolytic enzyme. An identical organisation could be detected in a genomic clone carrying a gene that encodes a protein kinase homologue in Arabidopsis thaliana (Hayashida et al., Gene, 121 (1992) 325-330). Southern and RT-PCR analyses indicate that Stpk1 is a single copy gene which is expressed throughout the plant with an increase in transcript abundance in leaf after infection with Phytophthora infestans. While comparisons using the deduced amino acid sequence for STPK1 indicate that it is most closely related to cyclic nucleotide-dependent protein kinases, it contains an additional plant-specific insert of 85 amino acids in the catalytic domain.

    Original languageEnglish
    Pages (from-to)71-80
    Number of pages10
    JournalPlant Science
    Volume118
    Issue number1
    DOIs
    Publication statusPublished - 21 Jul 1996

    Keywords

    • Gene linkage
    • Phytophthora infestans
    • Serine/threonine protein kinase
    • Synteny

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    • 2 Citations
    • 1 Comment/debate

    Erratum: Organisation and expression of a potato (Solanum tuberosum) protein kinase gene (Plant Science 118 (1996) 77)

    Meyer, R. C., Hedley, P. E., Waugh, R. & Machray, G. C., 1 Dec 1996, In : Plant Science. 120, 1, 1 p.

    Research output: Contribution to journalComment/debate

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