Organisation and expression of a potato (Solanum tuberosum) protein kinase gene

Rhonda C. Meyer; Peter E. Hedley; Robbie Waugh; Gordon C. Machray

doi:10.1016/0168-9452(96)04426-3

Organisation and expression of a potato (Solanum tuberosum) protein kinase gene

Rhonda C. Meyer (Lead / Corresponding author)
, Peter E. Hedley
, Robbie Waugh
, Gordon C. Machray

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A 4.3 kb genomic clone from potato (Solanum tuberosum) cv. Saturna has been isolated which contains the full coding sequence of a protein kinase gene (Stpk1), 1 kb of 3' sequence, and the first exon of a neighbouring gene encoding a sucrolytic enzyme. An identical organisation could be detected in a genomic clone carrying a gene that encodes a protein kinase homologue in Arabidopsis thaliana (Hayashida et al., Gene, 121 (1992) 325-330). Southern and RT-PCR analyses indicate that Stpk1 is a single copy gene which is expressed throughout the plant with an increase in transcript abundance in leaf after infection with Phytophthora infestans. While comparisons using the deduced amino acid sequence for STPK1 indicate that it is most closely related to cyclic nucleotide-dependent protein kinases, it contains an additional plant-specific insert of 85 amino acids in the catalytic domain.

Original language	English
Pages (from-to)	71-80
Number of pages	10
Journal	Plant Science
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(96)04426-3
Publication status	Published - 21 Jul 1996

Keywords

Gene linkage
Phytophthora infestans
Serine/threonine protein kinase
Synteny

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/0168-9452(96)04426-3

Cite this

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title = "Organisation and expression of a potato (Solanum tuberosum) protein kinase gene",

abstract = "A 4.3 kb genomic clone from potato (Solanum tuberosum) cv. Saturna has been isolated which contains the full coding sequence of a protein kinase gene (Stpk1), 1 kb of 3' sequence, and the first exon of a neighbouring gene encoding a sucrolytic enzyme. An identical organisation could be detected in a genomic clone carrying a gene that encodes a protein kinase homologue in Arabidopsis thaliana (Hayashida et al., Gene, 121 (1992) 325-330). Southern and RT-PCR analyses indicate that Stpk1 is a single copy gene which is expressed throughout the plant with an increase in transcript abundance in leaf after infection with Phytophthora infestans. While comparisons using the deduced amino acid sequence for STPK1 indicate that it is most closely related to cyclic nucleotide-dependent protein kinases, it contains an additional plant-specific insert of 85 amino acids in the catalytic domain.",

keywords = "Gene linkage, Phytophthora infestans, Serine/threonine protein kinase, Synteny",

author = "Meyer, \{Rhonda C.\} and Hedley, \{Peter E.\} and Robbie Waugh and Machray, \{Gordon C.\}",

year = "1996",

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TY - JOUR

T1 - Organisation and expression of a potato (Solanum tuberosum) protein kinase gene

AU - Meyer, Rhonda C.

AU - Hedley, Peter E.

AU - Waugh, Robbie

AU - Machray, Gordon C.

PY - 1996/7/21

Y1 - 1996/7/21

N2 - A 4.3 kb genomic clone from potato (Solanum tuberosum) cv. Saturna has been isolated which contains the full coding sequence of a protein kinase gene (Stpk1), 1 kb of 3' sequence, and the first exon of a neighbouring gene encoding a sucrolytic enzyme. An identical organisation could be detected in a genomic clone carrying a gene that encodes a protein kinase homologue in Arabidopsis thaliana (Hayashida et al., Gene, 121 (1992) 325-330). Southern and RT-PCR analyses indicate that Stpk1 is a single copy gene which is expressed throughout the plant with an increase in transcript abundance in leaf after infection with Phytophthora infestans. While comparisons using the deduced amino acid sequence for STPK1 indicate that it is most closely related to cyclic nucleotide-dependent protein kinases, it contains an additional plant-specific insert of 85 amino acids in the catalytic domain.

AB - A 4.3 kb genomic clone from potato (Solanum tuberosum) cv. Saturna has been isolated which contains the full coding sequence of a protein kinase gene (Stpk1), 1 kb of 3' sequence, and the first exon of a neighbouring gene encoding a sucrolytic enzyme. An identical organisation could be detected in a genomic clone carrying a gene that encodes a protein kinase homologue in Arabidopsis thaliana (Hayashida et al., Gene, 121 (1992) 325-330). Southern and RT-PCR analyses indicate that Stpk1 is a single copy gene which is expressed throughout the plant with an increase in transcript abundance in leaf after infection with Phytophthora infestans. While comparisons using the deduced amino acid sequence for STPK1 indicate that it is most closely related to cyclic nucleotide-dependent protein kinases, it contains an additional plant-specific insert of 85 amino acids in the catalytic domain.

KW - Gene linkage

KW - Phytophthora infestans

KW - Serine/threonine protein kinase

KW - Synteny

UR - https://www.scopus.com/pages/publications/0030596554

U2 - 10.1016/0168-9452(96)04426-3

DO - 10.1016/0168-9452(96)04426-3

M3 - Article

AN - SCOPUS:0030596554

SN - 0168-9452

VL - 118

SP - 71

EP - 80

JO - Plant Science

JF - Plant Science

IS - 1

ER -

Organisation and expression of a potato (Solanum tuberosum) protein kinase gene

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Erratum: Organisation and expression of a potato (Solanum tuberosum) protein kinase gene (Plant Science 118 (1996) 77)

Cite this