TY - JOUR
T1 - Orphan nuclear receptor RORα gene
T2 - Isoform-specific spatiotemporal expression during postnatal development of brain
AU - Sashihara, Shunsuke
AU - Felts, Paul A.
AU - Waxman, Stephen G.
AU - Matsui, Takashi
PY - 1996/11
Y1 - 1996/11
N2 - We analyzed expression of mouse orphan nuclear receptor RORα during postnatal development of rodent brain. Using a riboprobe corresponding to the 3'-end of mRORα cDNA, a peak of RORα: expression was observed at postnatal 16 day (P16) in the Purkinje cells of cerebellum, neurons of the thalamus and the olfactory bulb. The hippocampus was also shown to express RORα with an earlier peak at P7. Expression in cell types other than the Purkinje cells appeared transient. On the other hand, when a probe to the 5'-end of mRORα cDNA was used, we observed patterns of RORα expression that are different from those observed with the 3'-probe. No specific transcripts of RORα were detected with the 5'-probe in the Purkinje cells until P16. Additionally, the relative level of the hybridization signals with the 5'-probe and the 3'-probe were different among the various brain regions. Together with the previous findings that RORα comprises at least four isoforms which differ from one another in their N-terminal regions, these observations suggest that the spatiotemporal expression of RORα is under isoform-specific regulation. The timing of its expression suggests that RORα may be involved in regulation of postnatal maturation of specific classes of neurons.
AB - We analyzed expression of mouse orphan nuclear receptor RORα during postnatal development of rodent brain. Using a riboprobe corresponding to the 3'-end of mRORα cDNA, a peak of RORα: expression was observed at postnatal 16 day (P16) in the Purkinje cells of cerebellum, neurons of the thalamus and the olfactory bulb. The hippocampus was also shown to express RORα with an earlier peak at P7. Expression in cell types other than the Purkinje cells appeared transient. On the other hand, when a probe to the 5'-end of mRORα cDNA was used, we observed patterns of RORα expression that are different from those observed with the 3'-probe. No specific transcripts of RORα were detected with the 5'-probe in the Purkinje cells until P16. Additionally, the relative level of the hybridization signals with the 5'-probe and the 3'-probe were different among the various brain regions. Together with the previous findings that RORα comprises at least four isoforms which differ from one another in their N-terminal regions, these observations suggest that the spatiotemporal expression of RORα is under isoform-specific regulation. The timing of its expression suggests that RORα may be involved in regulation of postnatal maturation of specific classes of neurons.
KW - Neuron
KW - Orphan nuclear receptor
KW - Postnatal development
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0030298367&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(96)00118-0
DO - 10.1016/S0169-328X(96)00118-0
M3 - Article
C2 - 8915586
AN - SCOPUS:0030298367
VL - 42
SP - 109
EP - 117
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0169-328X
IS - 1
ER -