Oscillatory cAMP cell-cell signalling persists during multicellular Dictyostelium development

Gail Singer, Tsuyoshi Araki, Kees Weijer (Lead / Corresponding author)

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Abstract

Propagating waves of cAMP, periodically initiated in the aggregation centre, are known to guide the chemotactic aggregation of hundreds of thousands of starving individual Dictyostelium discoideum cells into multicellular aggregates. Propagating optical density waves, reflecting cell periodic movement, have previously been shown to exist in streaming aggregates, mounds and migrating slugs. Using a highly sensitive cAMP-FRET reporter, we have now been able to measure periodically propagating cAMP waves directly in these multicellular structures. In slugs cAMP waves are periodically initiated in the tip and propagate backward through the prespore zone. Altered cAMP signalling dynamics in mutants with developmental defects strongly support a key functional role for cAMP waves in multicellular Dictyostelium morphogenesis. These findings thus show that propagating cAMP not only control the initial aggregation process but continue to be the long range cell-cell communication mechanism guiding cell movement during multicellular Dictyostelium morphogenesis at the mound and slugs stages.
Original languageEnglish
Article number139
Pages (from-to)1-12
Number of pages12
JournalCommunications Biology
Volume2
Issue number1
DOIs
Publication statusPublished - 23 Apr 2019

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Gastropoda
Dictyostelium
Morphogenesis
Cell Movement
Cell Communication

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title = "Oscillatory cAMP cell-cell signalling persists during multicellular Dictyostelium development",
abstract = "Propagating waves of cAMP, periodically initiated in the aggregation centre, are known to guide the chemotactic aggregation of hundreds of thousands of starving individual Dictyostelium discoideum cells into multicellular aggregates. Propagating optical density waves, reflecting cell periodic movement, have previously been shown to exist in streaming aggregates, mounds and migrating slugs. Using a highly sensitive cAMP-FRET reporter, we have now been able to measure periodically propagating cAMP waves directly in these multicellular structures. In slugs cAMP waves are periodically initiated in the tip and propagate backward through the prespore zone. Altered cAMP signalling dynamics in mutants with developmental defects strongly support a key functional role for cAMP waves in multicellular Dictyostelium morphogenesis. These findings thus show that propagating cAMP not only control the initial aggregation process but continue to be the long range cell-cell communication mechanism guiding cell movement during multicellular Dictyostelium morphogenesis at the mound and slugs stages.",
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Oscillatory cAMP cell-cell signalling persists during multicellular Dictyostelium development. / Singer, Gail; Araki, Tsuyoshi; Weijer, Kees (Lead / Corresponding author).

In: Communications Biology, Vol. 2, No. 1, 139, 23.04.2019, p. 1-12.

Research output: Contribution to journalArticle

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