Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

Alaa A. El-Ghobashy, Abeer M. Shaaban, Jonathan Herod, Jenny Innes, Wendy Prime, C. Simon Herrington

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    Introduction. Cyclins are a family of regulatory proteins that play a
    pivotal role in controlling the cell cycle. While there is evidence of
    their altered expression in cervical squamous lesions, their precise
    role in glandular neoplasia is yet to be elucidated. Objectives. To
    investigate the role of cyclins as markers of early cervical glandular
    neoplasia by comparing their expression in lesions of different
    histological type. Methods. Through a cross-sectional analytical study,
    paraffin wax sections of normal cervix (n = 11),
    endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical
    glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive
    adenocarcinoma (n = 28) were studied using monoclonal antibodies for
    cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A
    quantitative assessment was employed for the analysis of percentage
    expression of each marker. Statistical analysis of data was performed
    using SPSS. Results. A progressive significant increase in cyclin A
    expression occurred from normal cervix (median: 0, IQ: 0-0), through
    endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30)
    to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B
    exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5,
    median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively).
    Statistically higher expression of cyclin B was found in CGIN than in
    TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed
    higher levels of cyclins A and B than CGIN (P < 0.001). There was
    significantly greater cyclin E expression in TEM/endometriosis than in
    normal cervix (P = 0.03) with a nonsignificant further increase in CGIN
    and invasive adenocarcinoma. The expression of cyclin D was not
    significantly different among all groups. Conclusions. Our data indicate
    that up-regulation of cyclin A and B expression occurs in neoplastic
    lesions of the cervix. Cyclin B expression was significantly more
    widespread in CGIN lesions than in TEM/endometriosis indicating that
    further assessment of the value of this marker in the diagnosis of
    cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All
    rights reserved.

    Original languageEnglish
    Pages (from-to)628-634
    Number of pages7
    JournalGynecologic Oncology
    Volume92
    Issue number2
    DOIs
    Publication statusPublished - 2004

    Cite this

    El-Ghobashy, A. A., Shaaban, A. M., Herod, J., Innes, J., Prime, W., & Herrington, C. S. (2004). Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. Gynecologic Oncology, 92(2), 628-634. https://doi.org/10.1016/j.ygyno.2003.11.010
    El-Ghobashy, Alaa A. ; Shaaban, Abeer M. ; Herod, Jonathan ; Innes, Jenny ; Prime, Wendy ; Herrington, C. Simon. / Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. In: Gynecologic Oncology. 2004 ; Vol. 92, No. 2. pp. 628-634.
    @article{71f3f6d0802e4e7d8e74b6d38d2d5698,
    title = "Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix",
    abstract = "Introduction. Cyclins are a family of regulatory proteins that play a pivotal role in controlling the cell cycle. While there is evidence of their altered expression in cervical squamous lesions, their precise role in glandular neoplasia is yet to be elucidated. Objectives. To investigate the role of cyclins as markers of early cervical glandular neoplasia by comparing their expression in lesions of different histological type. Methods. Through a cross-sectional analytical study, paraffin wax sections of normal cervix (n = 11), endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive adenocarcinoma (n = 28) were studied using monoclonal antibodies for cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A quantitative assessment was employed for the analysis of percentage expression of each marker. Statistical analysis of data was performed using SPSS. Results. A progressive significant increase in cyclin A expression occurred from normal cervix (median: 0, IQ: 0-0), through endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). Statistically higher expression of cyclin B was found in CGIN than in TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed higher levels of cyclins A and B than CGIN (P < 0.001). There was significantly greater cyclin E expression in TEM/endometriosis than in normal cervix (P = 0.03) with a nonsignificant further increase in CGIN and invasive adenocarcinoma. The expression of cyclin D was not significantly different among all groups. Conclusions. Our data indicate that up-regulation of cyclin A and B expression occurs in neoplastic lesions of the cervix. Cyclin B expression was significantly more widespread in CGIN lesions than in TEM/endometriosis indicating that further assessment of the value of this marker in the diagnosis of cervical glandular neoplasia is warranted. {\circledC} 2003 Elsevier Inc. All rights reserved.",
    author = "El-Ghobashy, {Alaa A.} and Shaaban, {Abeer M.} and Jonathan Herod and Jenny Innes and Wendy Prime and Herrington, {C. Simon}",
    year = "2004",
    doi = "10.1016/j.ygyno.2003.11.010",
    language = "English",
    volume = "92",
    pages = "628--634",
    journal = "Gynecologic Oncology",
    issn = "0090-8258",
    publisher = "Elsevier",
    number = "2",

    }

    El-Ghobashy, AA, Shaaban, AM, Herod, J, Innes, J, Prime, W & Herrington, CS 2004, 'Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix', Gynecologic Oncology, vol. 92, no. 2, pp. 628-634. https://doi.org/10.1016/j.ygyno.2003.11.010

    Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix. / El-Ghobashy, Alaa A.; Shaaban, Abeer M.; Herod, Jonathan; Innes, Jenny; Prime, Wendy; Herrington, C. Simon.

    In: Gynecologic Oncology, Vol. 92, No. 2, 2004, p. 628-634.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

    AU - El-Ghobashy, Alaa A.

    AU - Shaaban, Abeer M.

    AU - Herod, Jonathan

    AU - Innes, Jenny

    AU - Prime, Wendy

    AU - Herrington, C. Simon

    PY - 2004

    Y1 - 2004

    N2 - Introduction. Cyclins are a family of regulatory proteins that play a pivotal role in controlling the cell cycle. While there is evidence of their altered expression in cervical squamous lesions, their precise role in glandular neoplasia is yet to be elucidated. Objectives. To investigate the role of cyclins as markers of early cervical glandular neoplasia by comparing their expression in lesions of different histological type. Methods. Through a cross-sectional analytical study, paraffin wax sections of normal cervix (n = 11), endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive adenocarcinoma (n = 28) were studied using monoclonal antibodies for cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A quantitative assessment was employed for the analysis of percentage expression of each marker. Statistical analysis of data was performed using SPSS. Results. A progressive significant increase in cyclin A expression occurred from normal cervix (median: 0, IQ: 0-0), through endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). Statistically higher expression of cyclin B was found in CGIN than in TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed higher levels of cyclins A and B than CGIN (P < 0.001). There was significantly greater cyclin E expression in TEM/endometriosis than in normal cervix (P = 0.03) with a nonsignificant further increase in CGIN and invasive adenocarcinoma. The expression of cyclin D was not significantly different among all groups. Conclusions. Our data indicate that up-regulation of cyclin A and B expression occurs in neoplastic lesions of the cervix. Cyclin B expression was significantly more widespread in CGIN lesions than in TEM/endometriosis indicating that further assessment of the value of this marker in the diagnosis of cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All rights reserved.

    AB - Introduction. Cyclins are a family of regulatory proteins that play a pivotal role in controlling the cell cycle. While there is evidence of their altered expression in cervical squamous lesions, their precise role in glandular neoplasia is yet to be elucidated. Objectives. To investigate the role of cyclins as markers of early cervical glandular neoplasia by comparing their expression in lesions of different histological type. Methods. Through a cross-sectional analytical study, paraffin wax sections of normal cervix (n = 11), endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive adenocarcinoma (n = 28) were studied using monoclonal antibodies for cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A quantitative assessment was employed for the analysis of percentage expression of each marker. Statistical analysis of data was performed using SPSS. Results. A progressive significant increase in cyclin A expression occurred from normal cervix (median: 0, IQ: 0-0), through endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30) to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5, median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively). Statistically higher expression of cyclin B was found in CGIN than in TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed higher levels of cyclins A and B than CGIN (P < 0.001). There was significantly greater cyclin E expression in TEM/endometriosis than in normal cervix (P = 0.03) with a nonsignificant further increase in CGIN and invasive adenocarcinoma. The expression of cyclin D was not significantly different among all groups. Conclusions. Our data indicate that up-regulation of cyclin A and B expression occurs in neoplastic lesions of the cervix. Cyclin B expression was significantly more widespread in CGIN lesions than in TEM/endometriosis indicating that further assessment of the value of this marker in the diagnosis of cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All rights reserved.

    U2 - 10.1016/j.ygyno.2003.11.010

    DO - 10.1016/j.ygyno.2003.11.010

    M3 - Article

    VL - 92

    SP - 628

    EP - 634

    JO - Gynecologic Oncology

    JF - Gynecologic Oncology

    SN - 0090-8258

    IS - 2

    ER -