Overexpression of cyclins A and B as markers of neoplastic glandular lesions of the cervix

Alaa A. El-Ghobashy, Abeer M. Shaaban, Jonathan Herod, Jenny Innes, Wendy Prime, C. Simon Herrington

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    Abstract

    Introduction. Cyclins are a family of regulatory proteins that play a
    pivotal role in controlling the cell cycle. While there is evidence of
    their altered expression in cervical squamous lesions, their precise
    role in glandular neoplasia is yet to be elucidated. Objectives. To
    investigate the role of cyclins as markers of early cervical glandular
    neoplasia by comparing their expression in lesions of different
    histological type. Methods. Through a cross-sectional analytical study,
    paraffin wax sections of normal cervix (n = 11),
    endometriosis/tubo-endometrioid metaplasia (TEM) (n = 19), cervical
    glandular intraepithelial neoplasia (CGIN) (n = 33), and invasive
    adenocarcinoma (n = 28) were studied using monoclonal antibodies for
    cyclins A, B, D, and E with heat pretreatment for antigen unmasking. A
    quantitative assessment was employed for the analysis of percentage
    expression of each marker. Statistical analysis of data was performed
    using SPSS. Results. A progressive significant increase in cyclin A
    expression occurred from normal cervix (median: 0, IQ: 0-0), through
    endometriosis/TEM (median: 1, IQ: 0-15) and CGIN (median: 15, IQ: 0-30)
    to invasive adenocarcinoma (median: 40, IQ: 21.25-60). Cyclin B
    exhibited a similar pattern (median: 0, IQ: 0-0, median: 0, IQ: 0-0.5,
    median: 8, IQ: 0.75-15, and median: 30, IQ: 15-45, respectively).
    Statistically higher expression of cyclin B was found in CGIN than in
    TEM/endometriosis (P < 0.001). Invasive adenocarcinomas expressed
    higher levels of cyclins A and B than CGIN (P < 0.001). There was
    significantly greater cyclin E expression in TEM/endometriosis than in
    normal cervix (P = 0.03) with a nonsignificant further increase in CGIN
    and invasive adenocarcinoma. The expression of cyclin D was not
    significantly different among all groups. Conclusions. Our data indicate
    that up-regulation of cyclin A and B expression occurs in neoplastic
    lesions of the cervix. Cyclin B expression was significantly more
    widespread in CGIN lesions than in TEM/endometriosis indicating that
    further assessment of the value of this marker in the diagnosis of
    cervical glandular neoplasia is warranted. © 2003 Elsevier Inc. All
    rights reserved.

    Original languageEnglish
    Pages (from-to)628-634
    Number of pages7
    JournalGynecologic Oncology
    Volume92
    Issue number2
    DOIs
    Publication statusPublished - 2004

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