Overlapping functions of components of a bacterial Sec-independent protein export pathway

TY - JOUR

T1 - Overlapping functions of components of a bacterial Sec-independent protein export pathway

AU - Sargent, Frank

AU - Bogsch, Erik G.

AU - Stanley, Nicola R.

AU - Wexler, Margaret

AU - Robinson, Colin

AU - Berks, Ben C.

AU - Palmer, Tracy

PY - 1998

Y1 - 1998

N2 - We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.

AB - We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.

U2 - 10.1093/emboj/17.13.3640

DO - 10.1093/emboj/17.13.3640

M3 - Article

C2 - 9649434

SN - 0261-4189

VL - 17

SP - 3640

EP - 3650

JO - EMBO Journal

JF - EMBO Journal

IS - 13

ER -