Overview of molecular signatures of senescence and associated resources: pros and cons

Orestis A. Ntintas; Sylvia Vagena; Pavlos Pantelis; Giorgos Theocharous; Russel Petty; Konstantinos Evangelou; Vassilis G. Gorgoulis

doi:10.1002/2211-5463.70134

Overview of molecular signatures of senescence and associated resources: pros and cons

Orestis A. Ntintas
, Sylvia Vagena
, Pavlos Pantelis
, Giorgos Theocharous
, Russel Petty
, Konstantinos Evangelou
, Vassilis G. Gorgoulis (Lead / Corresponding author)

Cancer Research

Research output: Contribution to journal › Review article › peer-review

5 Citations (Scopus)

68 Downloads (Pure)

Abstract

The accurate detection of cellular senescence is of paramount importance given its involvement in aging and age-related pathologies. Over the years, a variety of markers and methodologies have been developed to address this issue. Initially, wet-lab assays, dealing with single morphological traits and molecular markers, were implemented, though exhibiting technical challenges and ineffectiveness in identifying the inherently complex senescence phenotype. Recent developments led to the adoption of combinatorial approaches in the form of multimarker guideline algorithms, effectively bypassing these obstacles. Moreover, technological advances have facilitated the emergence of molecular signatures that exploit the large amount of data generated in the last decades to increase our awareness of this phenomenon and its consequences. Due to the overwhelming expansion of these signatures, we performed an analysis of their advantages and disadvantages, and here, we discuss future improvements.

Original language	English
Pages (from-to)	821-836
Number of pages	16
Journal	FEBS Open Bio
Volume	16
Issue number	5
Early online date	4 Oct 2025
DOIs	https://doi.org/10.1002/2211-5463.70134
Publication status	Published - May 2026

Keywords

biomarkers
computational models
senescence
senescence molecular signatures

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology

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10.1002/2211-5463.70134Licence: CC BY

Final Published VersionFinal published version, 376 KBLicence: CC BY

Cite this

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title = "Overview of molecular signatures of senescence and associated resources: pros and cons",

abstract = "The accurate detection of cellular senescence is of paramount importance given its involvement in aging and age-related pathologies. Over the years, a variety of markers and methodologies have been developed to address this issue. Initially, wet-lab assays, dealing with single morphological traits and molecular markers, were implemented, though exhibiting technical challenges and ineffectiveness in identifying the inherently complex senescence phenotype. Recent developments led to the adoption of combinatorial approaches in the form of multimarker guideline algorithms, effectively bypassing these obstacles. Moreover, technological advances have facilitated the emergence of molecular signatures that exploit the large amount of data generated in the last decades to increase our awareness of this phenomenon and its consequences. Due to the overwhelming expansion of these signatures, we performed an analysis of their advantages and disadvantages, and here, we discuss future improvements.",

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AU - Ntintas, Orestis A.

AU - Vagena, Sylvia

AU - Pantelis, Pavlos

AU - Theocharous, Giorgos

AU - Petty, Russel

AU - Evangelou, Konstantinos

AU - Gorgoulis, Vassilis G.

PY - 2026/5

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N2 - The accurate detection of cellular senescence is of paramount importance given its involvement in aging and age-related pathologies. Over the years, a variety of markers and methodologies have been developed to address this issue. Initially, wet-lab assays, dealing with single morphological traits and molecular markers, were implemented, though exhibiting technical challenges and ineffectiveness in identifying the inherently complex senescence phenotype. Recent developments led to the adoption of combinatorial approaches in the form of multimarker guideline algorithms, effectively bypassing these obstacles. Moreover, technological advances have facilitated the emergence of molecular signatures that exploit the large amount of data generated in the last decades to increase our awareness of this phenomenon and its consequences. Due to the overwhelming expansion of these signatures, we performed an analysis of their advantages and disadvantages, and here, we discuss future improvements.

AB - The accurate detection of cellular senescence is of paramount importance given its involvement in aging and age-related pathologies. Over the years, a variety of markers and methodologies have been developed to address this issue. Initially, wet-lab assays, dealing with single morphological traits and molecular markers, were implemented, though exhibiting technical challenges and ineffectiveness in identifying the inherently complex senescence phenotype. Recent developments led to the adoption of combinatorial approaches in the form of multimarker guideline algorithms, effectively bypassing these obstacles. Moreover, technological advances have facilitated the emergence of molecular signatures that exploit the large amount of data generated in the last decades to increase our awareness of this phenomenon and its consequences. Due to the overwhelming expansion of these signatures, we performed an analysis of their advantages and disadvantages, and here, we discuss future improvements.

KW - biomarkers

KW - computational models

KW - senescence

KW - senescence molecular signatures

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Overview of molecular signatures of senescence and associated resources: pros and cons

Abstract

Keywords

ASJC Scopus subject areas

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