Oxysterol-induced cell death in U937 and HepG2 cells at reduced and normal serum concentrations

Y. C. O'Callaghan, J. A. Woods, N. M. O'Brien

    Research output: Contribution to journalArticle

    45 Citations (Scopus)

    Abstract

    Cholesterol oxidation products (oxysterols) are commonly found in foods of animal origin and are also produced endogenously in the body. Oxysterols are cytotoxic to certain cell lines and in some cases have been shown to induce apoptosis. The aim of this study was to investigate the effects of 7beta-hydroxy-cholesterol (7beta-OHC) and 25-hydroxycholesterol (25-OHC) on cytotoxicity and induction of apoptosis in U937 and HepG2 cells, treated in media containing either 2.5% foetal calf serum (FCS) or 10% FCS to examine the effect of increasing the cholesterol level.
    Original languageEnglish
    Pages (from-to)255-262
    Number of pages8
    JournalEuropean Journal of Nutrition
    Volume38
    Issue number6
    DOIs
    Publication statusPublished - 1999

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    U937 Cells
    Hep G2 Cells
    Cell Death
    Cholesterol
    Serum
    Apoptosis
    Cell Line
    Food
    Oxysterols

    Cite this

    O'Callaghan, Y. C. ; Woods, J. A. ; O'Brien, N. M. / Oxysterol-induced cell death in U937 and HepG2 cells at reduced and normal serum concentrations. In: European Journal of Nutrition. 1999 ; Vol. 38, No. 6. pp. 255-262.
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    abstract = "Cholesterol oxidation products (oxysterols) are commonly found in foods of animal origin and are also produced endogenously in the body. Oxysterols are cytotoxic to certain cell lines and in some cases have been shown to induce apoptosis. The aim of this study was to investigate the effects of 7beta-hydroxy-cholesterol (7beta-OHC) and 25-hydroxycholesterol (25-OHC) on cytotoxicity and induction of apoptosis in U937 and HepG2 cells, treated in media containing either 2.5{\%} foetal calf serum (FCS) or 10{\%} FCS to examine the effect of increasing the cholesterol level.",
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    Oxysterol-induced cell death in U937 and HepG2 cells at reduced and normal serum concentrations. / O'Callaghan, Y. C.; Woods, J. A.; O'Brien, N. M.

    In: European Journal of Nutrition, Vol. 38, No. 6, 1999, p. 255-262.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Oxysterol-induced cell death in U937 and HepG2 cells at reduced and normal serum concentrations

    AU - O'Callaghan, Y. C.

    AU - Woods, J. A.

    AU - O'Brien, N. M.

    PY - 1999

    Y1 - 1999

    N2 - Cholesterol oxidation products (oxysterols) are commonly found in foods of animal origin and are also produced endogenously in the body. Oxysterols are cytotoxic to certain cell lines and in some cases have been shown to induce apoptosis. The aim of this study was to investigate the effects of 7beta-hydroxy-cholesterol (7beta-OHC) and 25-hydroxycholesterol (25-OHC) on cytotoxicity and induction of apoptosis in U937 and HepG2 cells, treated in media containing either 2.5% foetal calf serum (FCS) or 10% FCS to examine the effect of increasing the cholesterol level.

    AB - Cholesterol oxidation products (oxysterols) are commonly found in foods of animal origin and are also produced endogenously in the body. Oxysterols are cytotoxic to certain cell lines and in some cases have been shown to induce apoptosis. The aim of this study was to investigate the effects of 7beta-hydroxy-cholesterol (7beta-OHC) and 25-hydroxycholesterol (25-OHC) on cytotoxicity and induction of apoptosis in U937 and HepG2 cells, treated in media containing either 2.5% foetal calf serum (FCS) or 10% FCS to examine the effect of increasing the cholesterol level.

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    DO - 10.1007/s003940050075

    M3 - Article

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    VL - 38

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    JO - European Journal of Nutrition

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