P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2

Dimitris P. Xirodimas, June Chisholm, Joana M. S. Desterro, David P. Lane, Ronald T. Hay, Julio Celis (Editor)

    Research output: Contribution to journalArticle

    79 Citations (Scopus)

    Abstract

    p14ARF tumour suppressor stabilises and activates p53 by directly interacting with (H)Mdm2 [(human) murine double minute 2 homologue] and inhibiting its E3 ubiquitin ligase activity. Here we demonstrate that p14ARF promotes accumulation of (H)Mdm2 conjugated to the small ubiquitin-like protein SUMO-1. Mutational analysis demonstrated that the N-terminus of Mdm2 is a target for p14ARF-mediated SUMO conjugation. SUMO modification requires residues 2–14 in p14ARF that interact with (H)Mdm2 and residues 82–101 in exon 2 involved in nucleolar localisation of p14ARF. These data suggest a novel role for p14ARF as a regulator of activity of (H)Mdm2, which could be related to its tumour suppressing activities.
    Original languageEnglish
    Pages (from-to)207-211
    Number of pages5
    JournalFEBS Letters
    Volume528
    Issue number1-3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Tumor Suppressor Protein p14ARF
    Tumors
    Ubiquitins
    Sumoylation
    Ubiquitin-Protein Ligases
    Exons
    Neoplasms

    Keywords

    • Mdm2
    • Hdm2

    Cite this

    Xirodimas, D. P., Chisholm, J., Desterro, J. M. S., Lane, D. P., Hay, R. T., & Celis, J. (Ed.) (2002). P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2. FEBS Letters, 528(1-3), 207-211. https://doi.org/10.1016/S0014-5793(02)03310-0
    Xirodimas, Dimitris P. ; Chisholm, June ; Desterro, Joana M. S. ; Lane, David P. ; Hay, Ronald T. ; Celis, Julio (Editor). / P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2. In: FEBS Letters. 2002 ; Vol. 528, No. 1-3. pp. 207-211.
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    abstract = "p14ARF tumour suppressor stabilises and activates p53 by directly interacting with (H)Mdm2 [(human) murine double minute 2 homologue] and inhibiting its E3 ubiquitin ligase activity. Here we demonstrate that p14ARF promotes accumulation of (H)Mdm2 conjugated to the small ubiquitin-like protein SUMO-1. Mutational analysis demonstrated that the N-terminus of Mdm2 is a target for p14ARF-mediated SUMO conjugation. SUMO modification requires residues 2–14 in p14ARF that interact with (H)Mdm2 and residues 82–101 in exon 2 involved in nucleolar localisation of p14ARF. These data suggest a novel role for p14ARF as a regulator of activity of (H)Mdm2, which could be related to its tumour suppressing activities.",
    keywords = "Mdm2, Hdm2",
    author = "Xirodimas, {Dimitris P.} and June Chisholm and Desterro, {Joana M. S.} and Lane, {David P.} and Hay, {Ronald T.} and Julio Celis",
    note = "dc.publisher: Elsevier dc.description.sponsorship: We would like to thank Drs K. Vousden and C. Sherr for providing us with reagents and Ellis Jaffray for purified SAE1/SAE2, Ubc9 and SUMO-1. This work was funded by Cancer Research UK. D.P.L. is a Gibb fellow of Cancer Research UK.",
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    Xirodimas, DP, Chisholm, J, Desterro, JMS, Lane, DP, Hay, RT & Celis, J (ed.) 2002, 'P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2', FEBS Letters, vol. 528, no. 1-3, pp. 207-211. https://doi.org/10.1016/S0014-5793(02)03310-0

    P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2. / Xirodimas, Dimitris P.; Chisholm, June; Desterro, Joana M. S.; Lane, David P.; Hay, Ronald T.; Celis, Julio (Editor).

    In: FEBS Letters, Vol. 528, No. 1-3, 2002, p. 207-211.

    Research output: Contribution to journalArticle

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    AU - Xirodimas, Dimitris P.

    AU - Chisholm, June

    AU - Desterro, Joana M. S.

    AU - Lane, David P.

    AU - Hay, Ronald T.

    A2 - Celis, Julio

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    Xirodimas DP, Chisholm J, Desterro JMS, Lane DP, Hay RT, Celis J, (ed.). P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2. FEBS Letters. 2002;528(1-3):207-211. https://doi.org/10.1016/S0014-5793(02)03310-0