P14ARF promotes accumulation of SUMO-1 conjugated (H)Mdm2

Dimitris P. Xirodimas, June Chisholm, Joana M. S. Desterro, David P. Lane, Ronald T. Hay, Julio Celis (Editor)

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    85 Citations (Scopus)


    p14ARF tumour suppressor stabilises and activates p53 by directly interacting with (H)Mdm2 [(human) murine double minute 2 homologue] and inhibiting its E3 ubiquitin ligase activity. Here we demonstrate that p14ARF promotes accumulation of (H)Mdm2 conjugated to the small ubiquitin-like protein SUMO-1. Mutational analysis demonstrated that the N-terminus of Mdm2 is a target for p14ARF-mediated SUMO conjugation. SUMO modification requires residues 2–14 in p14ARF that interact with (H)Mdm2 and residues 82–101 in exon 2 involved in nucleolar localisation of p14ARF. These data suggest a novel role for p14ARF as a regulator of activity of (H)Mdm2, which could be related to its tumour suppressing activities.
    Original languageEnglish
    Pages (from-to)207-211
    Number of pages5
    JournalFEBS Letters
    Issue number1-3
    Publication statusPublished - 2002


    • Mdm2
    • Hdm2


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