p21-LacZ reporter mice reflect p53-dependent toxic insult

Douglas B. Vasey; C. Roland Wolf; Thomas MacArtney; Ken Brown; C. Bruce A. Whitelaw

doi:10.1016/j.taap.2007.11.029

p21-LacZ reporter mice reflect p53-dependent toxic insult

Douglas B. Vasey, C. Roland Wolf, Thomas MacArtney, Ken Brown, C. Bruce A. Whitelaw

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity. (c) 2007 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	440-450
Number of pages	11
Journal	Toxicology and Applied Pharmacology
Volume	227
Issue number	3
DOIs	https://doi.org/10.1016/j.taap.2007.11.029
Publication status	Published - 15 Mar 2008

Keywords

cell cycle
drug
etoposide
hippocampus
toxicity
transgenic mice
KINASE INHIBITOR GENE
CELL-CYCLE ARREST
TRANSGENIC MICE
P53-INDEPENDENT PATHWAY
INDEPENDENT EXPRESSION
P21(WAF1) EXPRESSION
TUMOR-SUPPRESSOR
P21 WAF1/CIP1
DNA-DAMAGE
DIFFERENTIATION

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10.1016/j.taap.2007.11.029

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title = "p21-LacZ reporter mice reflect p53-dependent toxic insult",

abstract = "There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity. (c) 2007 Elsevier Inc. All rights reserved.",

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AU - Vasey, Douglas B.

AU - Wolf, C. Roland

AU - MacArtney, Thomas

AU - Brown, Ken

AU - Whitelaw, C. Bruce A.

PY - 2008/3/15

Y1 - 2008/3/15

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AB - There is an urgent need to discover less toxic and more selective drugs to treat disease. The use of transgenic mice that report on toxic insult-induced transcription can provide a valuable tool in this regard. To exemplify this strategy, we have generated transgenic mice carrying a p21-LacZ transgene. Transgene activity reflected endogenous p21 gene activation in various tissues, displayed compound-specific spatial expression signatures in the brain and immune tissues and enabled p53-dependent and p53-independent responses to be identified. We discuss the application of these mice in delineating the molecular events in normal cellular growth and disease and for the evaluation of drug toxicity. (c) 2007 Elsevier Inc. All rights reserved.

KW - cell cycle

KW - drug

KW - etoposide

KW - hippocampus

KW - toxicity

KW - transgenic mice

KW - KINASE INHIBITOR GENE

KW - CELL-CYCLE ARREST

KW - TRANSGENIC MICE

KW - P53-INDEPENDENT PATHWAY

KW - INDEPENDENT EXPRESSION

KW - P21(WAF1) EXPRESSION

KW - TUMOR-SUPPRESSOR

KW - P21 WAF1/CIP1

KW - DNA-DAMAGE

KW - DIFFERENTIATION

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DO - 10.1016/j.taap.2007.11.029

M3 - Article

C2 - 18215733

SN - 0041-008X

VL - 227

SP - 440

EP - 450

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

IS - 3

ER -

p21-LacZ reporter mice reflect p53-dependent toxic insult

Abstract

Keywords

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