p38 MAPK regulates COPII recruitment

Lijun Wang; John M. Lucocq

doi:10.1016/j.bbrc.2007.08.175

p38 MAPK regulates COPII recruitment

Lijun Wang, John M. Lucocq

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Here, we investigate regulation of coat protein complex II (COPII) recruitment onto ER export sites in permeabilized cells. In cytosols from nocodazole treated HeLa cells we find COPII loading is inhibited. The stress kinase p38 MAPK is activated in these cytosols and COPII loading can be rescued by depletion of p38 MAPK alpha or by the p38 MAPK inhibitor (SB203580) but not by inhibition/depletion of cdc2. These observations indicate regulation of the early secretory pathway by p38 MAPK.

Original language	English
Pages (from-to)	317-21
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	363
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.08.175
Publication status	Published - 16 Nov 2007

Keywords

COP-Coated Vesicles
Endoplasmic Reticulum
HeLa Cells
Humans
p38 Mitogen-Activated Protein Kinases

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10.1016/j.bbrc.2007.08.175

http://www.sciencedirect.com/science/article/pii/S0006291X07018906

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title = "p38 MAPK regulates COPII recruitment",

abstract = "Here, we investigate regulation of coat protein complex II (COPII) recruitment onto ER export sites in permeabilized cells. In cytosols from nocodazole treated HeLa cells we find COPII loading is inhibited. The stress kinase p38 MAPK is activated in these cytosols and COPII loading can be rescued by depletion of p38 MAPK alpha or by the p38 MAPK inhibitor (SB203580) but not by inhibition/depletion of cdc2. These observations indicate regulation of the early secretory pathway by p38 MAPK.",

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AU - Lucocq, John M.

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AB - Here, we investigate regulation of coat protein complex II (COPII) recruitment onto ER export sites in permeabilized cells. In cytosols from nocodazole treated HeLa cells we find COPII loading is inhibited. The stress kinase p38 MAPK is activated in these cytosols and COPII loading can be rescued by depletion of p38 MAPK alpha or by the p38 MAPK inhibitor (SB203580) but not by inhibition/depletion of cdc2. These observations indicate regulation of the early secretory pathway by p38 MAPK.

KW - COP-Coated Vesicles

KW - Endoplasmic Reticulum

KW - HeLa Cells

KW - Humans

KW - p38 Mitogen-Activated Protein Kinases

U2 - 10.1016/j.bbrc.2007.08.175

DO - 10.1016/j.bbrc.2007.08.175

M3 - Article

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VL - 363

SP - 317

EP - 321

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

p38 MAPK regulates COPII recruitment

Abstract

Keywords

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