TY - JOUR
T1 - p38/RK is essential for stress-induced nuclear responses
T2 - JNK/SAPKs and c-Jun/ATF-2 phosphorylation are insufficient
AU - Hazzalin, Catherine A.
AU - Cano, Eva
AU - Cuenda, Ana
AU - Barratt, Michael J.
AU - Cohen, Philip
AU - Mahadevan, Louis C.
PY - 1996/8
Y1 - 1996/8
N2 - The ERK, JNK/SAPK and p38/RK MAP kinase subtypes (reviewed in [1]) are differentially activated in mammalian cells by various stimuli, which elicit induction of immediate-early (IE) genes, such as c-fos and c-jun (reviewed in [1-3]), as well as phosphorylation of histone H3 [4] and HMG-14 [5]. Anisomycin and UV radiation have been suggested to induce c-fos and c-jun transcription via JNK/SAPK-mediated phosphorylation of TCF (ternary complex factor), for c-fos induction [6-8], and c-jun and/or ATF-2 for c-jun induction [9-13]. We report here that anisomycin and ultraviolet radiation (UV) activate MAP kinase kinase-6 (MKK6) [14,15], p38/RK [16-18] and MAPKAP kinase-2 (MAPKAP K-2) [17-19]. By using the p38/RK inhibitor SB 203580 [20,21], we show that activation of p38/RK and/or its downstream effectors are essential for anisomycin- and UV-stimulated c-fos/c-jun induction and histone H3/HMG-14 phosphorylation, whereas JNK/SAPK activation and phosphorylation of c-jun and ATF-2 are insufficient for these responses.
AB - The ERK, JNK/SAPK and p38/RK MAP kinase subtypes (reviewed in [1]) are differentially activated in mammalian cells by various stimuli, which elicit induction of immediate-early (IE) genes, such as c-fos and c-jun (reviewed in [1-3]), as well as phosphorylation of histone H3 [4] and HMG-14 [5]. Anisomycin and UV radiation have been suggested to induce c-fos and c-jun transcription via JNK/SAPK-mediated phosphorylation of TCF (ternary complex factor), for c-fos induction [6-8], and c-jun and/or ATF-2 for c-jun induction [9-13]. We report here that anisomycin and ultraviolet radiation (UV) activate MAP kinase kinase-6 (MKK6) [14,15], p38/RK [16-18] and MAPKAP kinase-2 (MAPKAP K-2) [17-19]. By using the p38/RK inhibitor SB 203580 [20,21], we show that activation of p38/RK and/or its downstream effectors are essential for anisomycin- and UV-stimulated c-fos/c-jun induction and histone H3/HMG-14 phosphorylation, whereas JNK/SAPK activation and phosphorylation of c-jun and ATF-2 are insufficient for these responses.
UR - http://www.scopus.com/inward/record.url?scp=0030220456&partnerID=8YFLogxK
U2 - 10.1016/S0960-9822(02)00649-8
DO - 10.1016/S0960-9822(02)00649-8
M3 - Article
C2 - 8805335
AN - SCOPUS:0030220456
VL - 6
SP - 1028
EP - 1031
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 8
ER -