p53 codon 72 ARG/PRO polymorphism is not related to HPV type or lesion grade in low- and high-grade squamous intra-epithelial lesions and invasive squamous carcinoma of the cervix

Athina Giannoudis, David A. Graham, Shirley A. Southern, C. Simon Herrington

    Research output: Contribution to journalArticle

    47 Citations (Scopus)

    Abstract

    A polymorphism at codon 72 of the p53 gene, resulting in either an arginine or a proline residue in the protein, has been reported to affect the susceptibility of p53 to human papillomavirus (HPV) E6-mediated degradation in cultured cells. However, the relevance of this polymorphism to naturally occurring HPV infection is unclear. Therefore, we analysed its relationship to infecting HPV type and lesion grade in a series of low- and high-grade squamous intra-epithelial lesions (SILs) and invasive carcinoma of the cervix. DNA extracted from morphologically characterised, paraffin-embedded tissues (30 normal cervices, 118 low-grade SILs, 118 high-grade SILs and 43 invasive carcinomas) was examined for the presence and type of HPV DNA, and the p53 genotype was identified by both allele-specific PCR and POP-restriction fragment length polymorphism. There was no significant relationship between the frequency of p53 genotypes and either HPV type or lesion grade. Our data do not support the hypothesis that this p53 polymorphism is involved in the development of high-grade squamous cervical disease in this population, (C) 1999 Wiley-Liss, Inc.

    Original languageEnglish
    Pages (from-to)66-69
    Number of pages4
    JournalInternational Journal of Cancer
    Volume83
    Issue number1
    DOIs
    Publication statusPublished - 1999

    Cite this

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    title = "p53 codon 72 ARG/PRO polymorphism is not related to HPV type or lesion grade in low- and high-grade squamous intra-epithelial lesions and invasive squamous carcinoma of the cervix",
    abstract = "A polymorphism at codon 72 of the p53 gene, resulting in either an arginine or a proline residue in the protein, has been reported to affect the susceptibility of p53 to human papillomavirus (HPV) E6-mediated degradation in cultured cells. However, the relevance of this polymorphism to naturally occurring HPV infection is unclear. Therefore, we analysed its relationship to infecting HPV type and lesion grade in a series of low- and high-grade squamous intra-epithelial lesions (SILs) and invasive carcinoma of the cervix. DNA extracted from morphologically characterised, paraffin-embedded tissues (30 normal cervices, 118 low-grade SILs, 118 high-grade SILs and 43 invasive carcinomas) was examined for the presence and type of HPV DNA, and the p53 genotype was identified by both allele-specific PCR and POP-restriction fragment length polymorphism. There was no significant relationship between the frequency of p53 genotypes and either HPV type or lesion grade. Our data do not support the hypothesis that this p53 polymorphism is involved in the development of high-grade squamous cervical disease in this population, (C) 1999 Wiley-Liss, Inc.",
    author = "Athina Giannoudis and Graham, {David A.} and Southern, {Shirley A.} and Herrington, {C. Simon}",
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    p53 codon 72 ARG/PRO polymorphism is not related to HPV type or lesion grade in low- and high-grade squamous intra-epithelial lesions and invasive squamous carcinoma of the cervix. / Giannoudis, Athina; Graham, David A.; Southern, Shirley A.; Herrington, C. Simon.

    In: International Journal of Cancer, Vol. 83, No. 1, 1999, p. 66-69.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Giannoudis, Athina

    AU - Graham, David A.

    AU - Southern, Shirley A.

    AU - Herrington, C. Simon

    PY - 1999

    Y1 - 1999

    N2 - A polymorphism at codon 72 of the p53 gene, resulting in either an arginine or a proline residue in the protein, has been reported to affect the susceptibility of p53 to human papillomavirus (HPV) E6-mediated degradation in cultured cells. However, the relevance of this polymorphism to naturally occurring HPV infection is unclear. Therefore, we analysed its relationship to infecting HPV type and lesion grade in a series of low- and high-grade squamous intra-epithelial lesions (SILs) and invasive carcinoma of the cervix. DNA extracted from morphologically characterised, paraffin-embedded tissues (30 normal cervices, 118 low-grade SILs, 118 high-grade SILs and 43 invasive carcinomas) was examined for the presence and type of HPV DNA, and the p53 genotype was identified by both allele-specific PCR and POP-restriction fragment length polymorphism. There was no significant relationship between the frequency of p53 genotypes and either HPV type or lesion grade. Our data do not support the hypothesis that this p53 polymorphism is involved in the development of high-grade squamous cervical disease in this population, (C) 1999 Wiley-Liss, Inc.

    AB - A polymorphism at codon 72 of the p53 gene, resulting in either an arginine or a proline residue in the protein, has been reported to affect the susceptibility of p53 to human papillomavirus (HPV) E6-mediated degradation in cultured cells. However, the relevance of this polymorphism to naturally occurring HPV infection is unclear. Therefore, we analysed its relationship to infecting HPV type and lesion grade in a series of low- and high-grade squamous intra-epithelial lesions (SILs) and invasive carcinoma of the cervix. DNA extracted from morphologically characterised, paraffin-embedded tissues (30 normal cervices, 118 low-grade SILs, 118 high-grade SILs and 43 invasive carcinomas) was examined for the presence and type of HPV DNA, and the p53 genotype was identified by both allele-specific PCR and POP-restriction fragment length polymorphism. There was no significant relationship between the frequency of p53 genotypes and either HPV type or lesion grade. Our data do not support the hypothesis that this p53 polymorphism is involved in the development of high-grade squamous cervical disease in this population, (C) 1999 Wiley-Liss, Inc.

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